Analysis of the mitochondrial maxicircle of Trypanosoma lewisi, a neglected human pathogen

0453402 - BC 2016 RIV GB eng J - Článek v odborném periodiku
Lin, R.-H. - Lai, D.-H. - Zheng, L.-L. - Wu, J. - Lukeš, Julius - Hide, G. - Lun, Z.-R.
Analysis of the mitochondrial maxicircle of Trypanosoma lewisi, a neglected human pathogen.
Parasites & Vectors. Roč. 8, 30 December 2015 (2015), s. 665. ISSN 1756-3305. E-ISSN 1756-3305
Institucionální podpora: RVO:60077344
Klíčová slova: Trypanosoma lewisi * Kinetoplast maxicircle * Mitochondrial DNA * RNA editing * Palindrome
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 3.234, rok: 2015

In this study, purified kinetoplast DNA from T. lewisi was sequenced using high-throughput sequencing in combination with sequencing of PCR amplicons. This allowed the assembly of the T. lewisi kinetoplast maxicircle DNA, which is a homologue of the mitochondrial genome in other eukaryotes. The assembly of 23,745 bp comprises the non-coding and coding regions. Comparative analysis of the maxicircle sequence of T. lewisi with Trypanosoma cruzi, Trypanosoma rangeli, Trypanosoma brucei and Leishmania tarentolae revealed that it shares 78 %, 77 %, 74 % and 66 % sequence identity with these parasites, respectively. The high GC content in at least 9 maxicircle genes of T. lewisi (ATPase6; NADH dehydrogenase subunits ND3, ND7, ND8 and ND9; G-rich regions GR3 and GR4; cytochrome oxidase subunit COIII and ribosomal protein RPS12) implies that their products may be extensively edited. A detailed analysis of the non-coding region revealed that it contains numerous repeat motifs and palindromes.
Trvalý link: http://hdl.handle.net/11104/0254237