0438211 - ÚOCHB 2015 RIV US eng J - Článek v odborném periodiku
Dziuba, Dmytro - Pohl, Radek - Hocek, Michal
Bodipy-Labeled Nucleoside Triphosphates for Polymerase Synthesis of Fluorescent DNA.
Bioconjugate Chemistry. Roč. 25, č. 11 (2014), s. 1984-1995. ISSN 1043-1802. E-ISSN 1520-4812
Grant CEP: GA ČR GBP206/12/G151
Institucionální podpora: RVO:61388963
Klíčová slova: enzyme amplification reaction * thymidine nucleotide analog * living cells
Kód oboru RIV: CC - Organická chemie
Impakt faktor: 4.513, rok: 2014 ; AIS: 1.195, rok: 2014
DOI: https://doi.org/10.1021/bc5003554

New fluorescent nucleosides and nucleoside triphosphate (dNTPs) analogs bearing the F-Bodipy fluorophore linked through a short, flexible nonconjugate tether were synthesized. The Bodipy-labeled dNTPs were substrates for several DNA polymerases which incorporated them into DNA in primer extension, nicking enzyme amplification reaction, and polymerase chain reaction. The fluorescence of F-Bodipy is not quenched upon incorporation in DNA and can be detected both in solutions and on gels.
Trvalý link: http://hdl.handle.net/11104/0241676