beta-Arrestin Interacts with the Beta/Gamma Subunits of Trimeric G-Proteins and Dishevelled in the Wnt/Ca2+ Pathway in Xenopus Gastrulation

0427975 - BFÚ 2015 RIV US eng J - Článek v odborném periodiku
Seitz, K. - Dursch, V. - Harnoš, J. - Bryja, Vítězslav - Gentzel, M. - Schambony, A.
beta-Arrestin Interacts with the Beta/Gamma Subunits of Trimeric G-Proteins and Dishevelled in the Wnt/Ca2+ Pathway in Xenopus Gastrulation.
PLoS ONE. Roč. 9, č. 1 (2014). ISSN 1932-6203. E-ISSN 1932-6203
Grant CEP: GA ČR(CZ) GC204/09/J030
Grant ostatní: GA ČR(CZ) GA204/09/0498
Institucionální podpora: RVO:68081707
Klíčová slova: CONVERGENT EXTENSION MOVEMENTS * WNT SIGNALING PATHWAYS * WNT/BETA-CATENIN
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 3.234, rok: 2014

beta-Catenin independent, non-canonical Wnt signaling pathways play a major role in the regulation of morphogenetic movements in vertebrates. The term non-canonical Wnt signaling comprises multiple, intracellularly divergent, Wnt-activated and beta-Catenin independent signaling cascades including the Wnt/Planar Cell Polarity and the Wnt/Ca2+ cascades. Wnt/Planar Cell Polarity and Wnt/Ca2+ pathways share common effector proteins, including the Wnt ligand, Frizzled receptors and Dishevelled, with each other and with additional branches of Wnt signaling. Along with the aforementioned proteins, beta-Arrestin has been identified as an essential effector protein in the Wnt/beta-Catenin and the Wnt/Planar Cell Polarity pathway. Our results demonstrate that beta-Arrestin is required in the Wnt/Ca2+ signaling cascade upstream of Protein Kinase C (PKC) and Ca2+/Calmodulin-dependent Protein Kinase II (CamKII). We have further characterized the role of beta-Arrestin in this branch of non-canonical Wnt signaling by knock-down and rescue experiments in Xenopus embryo explants and analyzed protein-protein interactions in 293T cells.
Trvalý link: http://hdl.handle.net/11104/0233414