Plant secondary metabolite-induced shifts in bacterial community structure and degradative ability in contaminated soil

0423263 - ÚMG 2014 RIV DE eng J - Článek v odborném periodiku
Uhlík, O. - Musilová, L. - Rídl, Jakub - Hroudová, Miluše - Vlček, Čestmír - Koubek, J. - Holečková, M. - Mackova, M. - Macek, T.
Plant secondary metabolite-induced shifts in bacterial community structure and degradative ability in contaminated soil.
Applied Microbiology and Biotechnology. Roč. 97, č. 20 (2013), s. 9245-9256. ISSN 0175-7598. E-ISSN 1432-0614
Grant ostatní: EK(XE) 265946; GA MŠk(CZ) ME10041
Institucionální podpora: RVO:68378050
Klíčová slova: plant secondary metabolites (PSM) * bacterial community * metabolic activity * bioremediation * pyrosequencing
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 3.811, rok: 2013

We investigated how selected natural compounds (naringin, caffeic acid, and limonene) induce shifts in bacterial community structure and degradative activity in long-term PCB-contaminated soil and how these changes correlate with changes in chlorobiphenyl degradation capacity. In order to address this issue, we have integrated analytical methods of determining PCB degradation with pyrosequencing of 16S rRNA gene tag-encoded amplicons and DNA-stable isotope probing (SIP). Our model system was set in laboratory microcosms with PCB-contaminated soil, which was enriched for 8 weeks with the suspensions of flavonoid naringin, terpene limonene, and phenolic caffeic acid. Our results show that application of selected plant secondary metabolites resulted in bacterial community structure far different from the control one (no natural compound amendment). The community in soil treated with caffeic acid is almost solely represented by Proteobacteria, Acidobacteria, and Verrucomicrobia (together over 99 %). Treatment with naringin resulted in enrichment of Firmicutes to the exclusion of Acidobacteria and Verrucomicrobia. SIP was applied to identifying populations actively participating in 4-chlorobiphenyl catabolism. Naringin and limonene in soil fostered mainly populations of Hydrogenophaga spp., caffeic acid Burkholderia spp. and Pseudoxanthomonas spp. None of these populations were detected among 4-chlorobiphenyl utilizers in non-amended soil. Similarly, the degradation of individual PCB congeners was influenced by addition of different plant compounds. Residual content of PCBs was lowest after treating the soil with naringin. Addition of caffeic acid resulted in comparable decrease of total PCBs with non-amended soil; however, higher substituted congeners were more degraded after caffeic acid treatment compared to all other treatments. Plant secondary metabolites seem to have a strong effect on the bacterial community structure, activity and associated degradative ability.
Trvalý link: http://hdl.handle.net/11104/0229381