Viability, longevity, and egg production of Drosophila melanogaster are regulated by the miR-282 microDNA

0422599 - BC 2014 RIV US eng J - Článek v odborném periodiku
Vilmos, P. - Bujna, Á. - Szuperák, M. - Havelda, Z. - Várallyay, É. - Szabad, J. - Kučerová, Lucie - Somogyi, K. - Kristó, I. - Lukácsovich, T. - Jankovics, F. - Henn, L. - Erdélyi, M.
Viability, longevity, and egg production of Drosophila melanogaster are regulated by the miR-282 microDNA.
Genetics. Roč. 195, č. 2 (2013), s. 469-480. ISSN 0016-6731. E-ISSN 1943-2631
Grant ostatní: Hungarian National Science Foundation(HU) NK84121; Hungarian National Science Foundation(HU) K108538
Institucionální podpora: RVO:60077344
Klíčová slova: Drosophila melanogaster
Kód oboru RIV: ED - Fyziologie
Impakt faktor: 4.866, rok: 2013

The first microRNAs were discovered some 20 years ago, but only a small fraction of the microRNA-encoding genes have been described in detail yet. Here we report the molecular analysis of a computationally predicted Drosophila melanogaster microRNA gene, mir-282. We show that the mir-282 gene is the source of a 4.9-kb-long primary transcript with a 5' cap and a 3'-poly(A) sequence and a mature microRNA of similar to 25 bp. Our data strongly suggest the existence of an independent mir-282 gene conserved in holometabolic insects. We give evidence that the mir-282 locus encodes a functional transcript that influences viability, longevity, and egg production in Drosophila. We identify the nervous system-specific adenylate cyclase (rutabaga) as a target of miR-282 and assume that one of the main functions of mir-282 is the regulation of adenylate cyclase activity in the nervous system during metamorphosis.
Trvalý link: http://hdl.handle.net/11104/0230602