Isoelectric focusing in continuously tapered fused silica capillary prepared by etching with supercritical water

0392235 - ÚIACH 2014 RIV US eng J - Článek v odborném periodiku
Šlais, Karel - Horká, Marie - Karásek, Pavel - Planeta, Josef - Roth, Michal
Isoelectric focusing in continuously tapered fused silica capillary prepared by etching with supercritical water.
Analytical Chemistry. Roč. 85, č. 9 (2013), s. 4296-4300. ISSN 0003-2700. E-ISSN 1520-6882
Grant CEP: GA ČR(CZ) GAP106/12/0522; GA MV VG20102015023
Institucionální podpora: RVO:68081715
Klíčová slova: capillary isoelectric focusing * resolution of ampholytes * supercritical water
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 5.825, rok: 2013

In capillary isoelectric focusing carried out in tapered capillaries, several times higher resolution of ampholytes can be obtained as compared with the resolution of the respective pair of ampholytes in conventional cylindrical capillaries. The tapered fused-silica capillaries were reproducibly prepared by etching with supercritical water, and they open the way to increase the number of separable compounds without resorting to excessively high voltage.
Trvalý link: http://hdl.handle.net/11104/0221156