Lipid alterations in human colon epithelial cells induced to differentiation and/or apoptosis by butyrate and polyunsaturated fatty acids

0389673 - BFÚ 2013 RIV US eng J - Článek v odborném periodiku
Hofmanová, Jiřina - Ciganek, M. - Slavík, J. - Kozubík, Alois - Stixová, Lenka - Vaculová, Alena - Dušek, L. - Machala, M.
Lipid alterations in human colon epithelial cells induced to differentiation and/or apoptosis by butyrate and polyunsaturated fatty acids.
Journal of Nutritional Biochemistry. Roč. 23, č. 6 (2012), s. 539-548. ISSN 0955-2863. E-ISSN 1873-4847
Grant CEP: GA ČR(CZ) GA524/07/1178; GA ČR(CZ) GAP301/11/1730
Výzkumný záměr: CEZ:AV0Z50040507
Institucionální podpora: RVO:68081707
Klíčová slova: Colon cancer * Polyunsaturated fatty acids * Butyrate
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 4.552, rok: 2012

The present study highlights the important association between lipid alterations and differentiation/apoptotic responses in human colon differentiating (FHC) and nondifferentiating (HCT-116) cell lines after their treatment with short-chain fatty acid sodium butyrate (NaBt), polyunsaturated fatty acids (PUFAs), and/or their combination. Our data from GC/MS and LC/MS/MS showed an effective incorporation and metabolization of the supplemented arachidonic acid (AA) or docosahexaenoic acid (DNA), resulting in an enhanced content of the respective PUFA in individual phospholipid (PL) classes and an altered composition of the whole cellular fatty acid spectrum in both FHC and HCT-116 cells. We provide novel evidence that NaBt combined with PUFAs additionally modulated AA and DHA cellular levels and caused their shift from triacylglycerol to PL fractions.
Trvalý link: http://hdl.handle.net/11104/0218526