Application of Colloidal Palladium Nanoparticles for Labeling in Electron Microscopy

0367418 - BC 2012 RIV US eng J - Článek v odborném periodiku
Vancová, Marie - Šlouf, Miroslav - Langhans, Jan - Pavlová, Eva - Nebesářová, Jana
Application of Colloidal Palladium Nanoparticles for Labeling in Electron Microscopy.
Microscopy and Microanalysis. Roč. 17, č. 5 (2011), s. 810-816. ISSN 1431-9276. E-ISSN 1435-8115
Grant CEP: GA AV ČR KAN200520704; GA AV ČR KJB600960906; GA ČR GAP205/10/0348
Výzkumný záměr: CEZ:AV0Z60220518; CEZ:AV0Z40500505
Klíčová slova: electron microscopy * colloidal palladium * nanoparticles * labeling * salivary glands * Ixodes ricinus
Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie
Impakt faktor: 3.007, rok: 2011

The application of palladium nanoparticles as electron-dense markers for labeling in both transmission and scanning electron microscopy requires their conjugation to a specific protein. The conjugation protocol described here includes the dihydrolipoic acid capping of Pd nanoparticles and their subsequent covalent attachment to protein molecules such as streptavidin, protein A, or avidin. The single-step reaction was mediated using the cross-linking agent ethyl-3-(3- dimethylaminopropyl)carbodiimide. The final Pd conjugates were fully functional, as demonstrated by labeling of ultrathin resin sections of either bovine serum albumin or secretory granules of the salivary gland isolated from the partially fed female Ixodes ricinus tick. The results were quantified, evaluated, and compared with results obtained using commercially available gold particle conjugates. Both the streptavidin-Pd conjugate and commercial streptavidin-Au conjugate were used to detect fucosylated glycans.
Trvalý link: http://hdl.handle.net/11104/0202116