Sequencing and assembly of low copy and genic regions of isolated Triticum aestivum chromosome arm 7DS

0365378 - ÚEB 2012 RIV US eng J - Článek v odborném periodiku
Berkman, O. J. - Skarshewski, A. - Lorenc, M. T. - Kubaláková, Marie - Šimková, Hana - Batley, J. - Doležel, Jaroslav - Edwards, D. … celkem 17 autorů
Sequencing and assembly of low copy and genic regions of isolated Triticum aestivum chromosome arm 7DS.
Plant Biotechnology Journal. Roč. 9, č. 7 (2011), s. 768-775. ISSN 1467-7644. E-ISSN 1467-7652
Grant CEP: GA ČR GA521/07/1573; GA MŠMT ED0007/01/01
Výzkumný záměr: CEZ:AV0Z50380511
Klíčová slova: wheat genome sequence * chromosome 7 * genome assembly
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 5.442, rok: 2011

The genome of bread wheat (Triticum aestivum) is predicted to be greater than 16 Gbp in size and consist predominantly of repetitive elements, making the sequencing and assembly of this genome a major challenge. We have reduced genome sequence complexity by isolating chromosome arm 7DS and applied second-generation technology and appropriate algorithmic analysis to sequence and assemble low copy and genic regions of this chromosome arm. The assembly represents approximately 40% of the chromosome arm and all known 7DS genes. Comparison of the 7DS assembly with the sequenced genomes of rice (Oryza sativa) and Brachypodium distachyon identified large regions of conservation. The syntenic relationship between wheat, B. distachyon and O. sativa, along with available genetic mapping data, has been used to produce an annotated draft 7DS syntenic build, which is publicly available at http://www.wheatgenome.info.
Trvalý link: http://hdl.handle.net/11104/0200633