Bioluminescence of Pseudomonas Fluorescens HK44 in the Course of Encapsulation into Silica Gel. Effect of Methanol

0359057 - ÚCHP 2012 RIV CZ eng J - Článek v odborném periodiku
Trögl, J. - Kuncová, Gabriela - Kuráň, P.
Bioluminescence of Pseudomonas Fluorescens HK44 in the Course of Encapsulation into Silica Gel. Effect of Methanol.
Folia Microbiologica. Roč. 55, č. 6 (2010), s. 569-575. ISSN 0015-5632. E-ISSN 1874-9356
Grant CEP: GA MŠMT ME 893
Výzkumný záměr: CEZ:AV0Z40720504
Klíčová slova: catabolic reporter bacterium * recombinant escheria-coli * optical biosensor
Kód oboru RIV: CE - Biochemie
Impakt faktor: 0.977, rok: 2010

The bioluminescence (BLM) and colony-forming units (CFU) of Pseudomonas fluorescens HK44 were monitored during encapsulation into pre-polymerized Si(OMe)(4). The non-induced BLM of free cells was increased in the presence of 0.5-2.5 % MeOH. After mixing silica sol with the cell suspension, both BLM and CFU dropped to 1-3 and 8-18 %, respectively; both remained lowered as long as the silica biofilm contained residual MeOH. The kinetics of MeOH being released from silica biofilms (a thickness of 2-6 mm) were first-order. The decrease of bacterial activity due to encapsulation was proportional to the biofilm thickness. MeOH evolving during encapsulation is probably the principal stress factor but not the only one.
Trvalý link: http://hdl.handle.net/11104/0196927