Assessing oestrogenic effects of brominated flame retardants Hexabromocyclododecane and Tetrabromobisphenol A on MCF-7 cells

0356209 - BTÚ 2012 RIV CZ eng J - Článek v odborném periodiku
Dorosh, Andriy - Děd, Lukáš - Elzeinová, Fatima - Pěknicová, Jana
Assessing oestrogenic effects of brominated flame retardants Hexabromocyclododecane and Tetrabromobisphenol A on MCF-7 cells.
Folia Biologica. Roč. 56, - (2010), s. 35-39. ISSN 0015-5500. E-ISSN 0015-5500
Grant CEP: GA MŠMT(CZ) 1M06011; GA MŠMT(CZ) 2B06151
Výzkumný záměr: CEZ:AV0Z50520701
Klíčová slova: endocrine disruptors * BRF - brominated flame retardant * MCF-7 cells * TFF1 - trefoil factor
Kód oboru RIV: DN - Vliv životního prostředí na zdraví
Impakt faktor: 0.729, rok: 2010

We used the proliferation test (E-screen assay) in MCF-7 breast cancer cells and reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis of TFF1 gene expression to analyze estrogenicity of the studied compounds. RT-qPCR has proven to be a fast and valuable molecular technique in gene expression quantification. HBCD but not TBBPA increased cell proliferation in MCF-7 cells and up-regulates TFF1 gene expression in a concentration-dependent manner. Anti-estrogen ICI 182,780 inhibits up-regulation of TFF1 by HBCD. We showed that HBCD displays estrogen-like effects on MCF-7 cells. TBBPA, on the other hand, has not shown any estrogenic effect mediated by the estrogen receptor alpha.
Trvalý link: http://hdl.handle.net/11104/0194791