Molecular detection of Entoloma spp. associated with roots of rosaceous woody plants

0350285 - MBÚ 2011 RIV DE eng J - Článek v odborném periodiku
Gryndler, Milan - Egertová, Z. - Soukupová, Lucie - Gryndlerová, Hana - Borovička, Jan - Hršelová, Hana
Molecular detection of Entoloma spp. associated with roots of rosaceous woody plants.
Mycological Progress. Roč. 9, č. 1 (2010), s. 27-36. ISSN 1617-416X. E-ISSN 1861-8952
Grant CEP: GA ČR GA526/06/0540
Výzkumný záměr: CEZ:AV0Z30130516; CEZ:AV0Z50200510
Klíčová slova: Fruit trees * Cellobiose * Inoculation
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 1.266, rok: 2010

Seventeen isolates of Entoloma clypeatum, a fungus associated with rosaceous woody plants and suspected to act as a root pathogen, were obtained from fruit-bodies collected at 7 localities of the Czech Republic. Culture conditions and sensitivity to fungicides have been studied. A PCR primer directed to LSU rRNA gene specific to Entoloma associated with rosaceous plants has been designed. This primer can be used to detect the fungus in cultivated rosaceous fruit trees. The method is robust towards the false positive detection caused by nonspecific amplification of DNA of other soil microorganisms
Trvalý link: http://hdl.handle.net/11104/0190322