Development of Chromosome-Specific BAC Resources for Genomics of Bread Wheat

0349181 - ÚEB 2011 RIV CH eng J - Článek v odborném periodiku
Šafář, Jan - Šimková, Hana - Kubaláková, Marie - Čihalíková, Jarmila - Suchánková, Pavla - Bartoš, Jan - Doležel, Jaroslav
Development of Chromosome-Specific BAC Resources for Genomics of Bread Wheat.
Cytogenetic and Genome Research. Roč. 129, 1-3 (2010), s. 211-223. ISSN 1424-8581. E-ISSN 1424-859X
Grant CEP: GA ČR GA521/07/1573; GA MŠMT(CZ) LC06004
Grant ostatní: European Community’s Seventh Framework Programme(XE) FP7/2007–2013
Výzkumný záměr: CEZ:AV0Z50380511
Klíčová slova: BAC library * Chromosome * DNA markers
Kód oboru RIV: GE - Šlechtění rostlin
Impakt faktor: 1.783, rok: 2010

The large bread wheat genome (1C similar to 17 Gbp) contains a preponderance of repetitive DNA and the species is polyploid. These characteristics together serve to hamper the molecular analysis of the wheat genome. Its complexity can, however, be reduced by using flow cytometry to isolate individual chromosomes, and these can be exploited to construct chromosome-specific BAC libraries. Such libraries simplify the task of physical map construction, positional cloning and the targeted development of genetic markers. Rapid improvements in the efficiency and cost of DNA sequencing provide an opportunity to contemplate sequencing the wheat genome by preparing sequence-ready physical maps for each chromosome or chromosome arm in turn. The quality of the chromosome-specific libraries depends on their chromosome coverage and the mean insert size. First-generation libraries suffered from a relatively low mean insert size.
Trvalý link: http://hdl.handle.net/11104/0189489