Molecular variability of FLT3/ITD mutants and their impact on the differentiation program of 32D cells: Implications for the biological properties of AML blasts

0333746 - ÚMG 2010 RIV GB eng J - Článek v odborném periodiku
Peková, S. - Ivánek, Robert - Dvořák, Michal - Rueggeberg, S. - Leicht, S. - Li, X. - Franz, T. - Kozak, T. - Vrba, J. - Koza, V. - Karas, M. - Schwarz, J. - Cetkovský, P. - Průcha, M.
Molecular variability of FLT3/ITD mutants and their impact on the differentiation program of 32D cells: Implications for the biological properties of AML blasts.
Leukemia Research. Roč. 33, č. 10 (2009), s. 1409-1416. ISSN 0145-2126. E-ISSN 1873-5835
Grant CEP: GA ČR GA204/06/1728
Výzkumný záměr: CEZ:AV0Z50520514
Klíčová slova: FLT3/ITD * microarrays * proteomics
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 2.358, rok: 2009

FLT3 is the most frequently mutated gene in acute myeloid leukemia (AML), with internal tandem duplications (ITDs) accounting for up to 30% of its mutations. To analyze the impact of individual ITDs on the expression profile of immature myeloid cells, we have established 32D cell lines expressing nine different FLT3/ITDs isolated from AML patients and subjected them to whole genome expression profiling and 2DE/LC/MS proteomics. Our data indicate that in comparison to the controls, FLT3/ITD-positive 32D cells exhibit less mature expression profiles resembling early hematopoietic progenitors. Moreover, our results suggest that there exist biological differences among individual ITD variants.
Trvalý link: http://hdl.handle.net/11104/0178666