Housekeeping gene selection for Real Time-PCR normalization in female hop (Humulus lupulus L) tissues

0330271 - BC 2010 RIV IN eng J - Článek v odborném periodiku
Maloukh, L. - Matoušek, Jaroslav - Van Bockstaele, E. - Roldán-Ruiz, I.
Housekeeping gene selection for Real Time-PCR normalization in female hop (Humulus lupulus L) tissues.
Journal of Plant Biochemistry and Biotechnology. Roč. 18, č. 1 (2009), s. 53-58. ISSN 0971-7811. E-ISSN 0974-1275
Výzkumný záměr: CEZ:AV0Z50510513
Klíčová slova: gene expression normalization * hop Humulus lupulus * Real Time PCR
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 0.323, rok: 2009

The variability of expression six genes was estimated in leaves and female inflorescences of two hop cultivars (White Golding and Admiral) at different developmental stages by RT-PCR. The combination of the three housekeeping genes DRH1, GAPDH and 7SL-RNA as internal standards for hop, provided reliable results in the quantitative analysis of the transcript accumulation of Hua Enhancer 1 transcription factor (HEN1) homologue in hop tissues and is recommended for future studies of gene expression in female hop tissues.
Trvalý link: http://hdl.handle.net/11104/0176100