0172386 - UEB-Q 20033094 RIV NL eng J - Článek v odborném periodiku
Kolářová, H. - Mosinger, J. - Lenobel, René - Kejlová, K. - Jírová, D. - Strnad, Miroslav
In vitro toxicity testing of supramolecular sensitizers for photodynamic therapy.
Toxicology in Vitro. Roč. 17, 5/6 (2003), s. 775-778. ISSN 0887-2333. E-ISSN 1879-3177
Grant CEP: GA ČR GA203/02/1483
Výzkumný záměr: CEZ:AV0Z5038910; CEZ:MSM 153100008
Klíčová slova: Sensitizers * Phototoxicity * Photodynamic therapy
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 1.642, rok: 2003

We report the phototoxicity of meso-tetrakis(4-sulphonatophenyl)porphine (TPPS4) and zinc metallocomplex (ZnTPPS4) sensitizers in the presence or absence of 2-hydroxypropyl-beta-cyclodextrin (HP-beta-CD) on G361human melanoma cells. Morphological changes in cell cultures have been evaluated using inversion fluorescent microscope and image analysis. Viability of cells was determined by means of molecular probes for fluorescence microscopy (LIVE/DEAD kit- double staining with Calcein AM and Ethidium Homodimer). The quantitative changes of cell viability in relation to sensitizers concentrations and irradiation doses were proved by fluorometric measurement with fluoroscan Ascent. We found that the most effective sensitizer is ZnTPPS4 bound to HP-beta-CD, since the IC50 value was 12.5 g/ml at the dose of light radiation of 10 J/cm2.
Trvalý link: http://hdl.handle.net/11104/0069425