0172236 - UEB-Q 20023013 RIV CZ eng J - Článek v odborném periodiku
Ajalin, I. - Kobza, F. - Doležel, Jaroslav
Ploidy identification of doubled chromosome number plants in Viola x wittrockiana Gams. M1-generation.
Horticultural Science. Roč. 29, č. 1 (2002), s. 35-40. ISSN 1416-6445
Grant CEP: GA AV ČR IAA6038204; GA AV ČR IBS5038104
Výzkumný záměr: CEZ:AV0Z5038910
Klíčová slova: flow cytometry * polyploidisation * colchicine
Kód oboru RIV: EB - Genetika a molekulární biologie

The aim of this study was to develop a protocol for production of polyploid Ml-generation plants of Viola x wittrockiana Gams. Two variants of colchicine treatment were compared for their efficiency. Early detection of novel ploidy levels was achieved by screening of stomata size, leaf index value (leaf blade length/width), and other morphological characteristics of the Ml-generation. Secondary screening for novel ploidy levels was performed by flow cytometry (FCM). Hexadecaploid, aneuploid, and mixoploid plants were successfully identified by FCM.
Trvalý link: http://hdl.handle.net/11104/0069275