0172223 - UEB-Q 20023005 RIV NL eng J - Článek v odborném periodiku
Dobrev, Petre - Kamínek, Miroslav
Fast and efficient separation of cytokinins from auxin and abscisic acid and their purification using mixed-mode solid-phase extraction.
Journal of Chromatography A. Roč. 950, č. 1 (2002), s. 21-29. ISSN 0021-9673. E-ISSN 1873-3778
Grant CEP: GA ČR GA206/02/0967; GA ČR GA522/02/0530
Výzkumný záměr: CEZ:AV0Z5038910
Klíčová slova: cytokinins * abscisic acid * extraction
Kód oboru RIV: EF - Botanika
Impakt faktor: 3.098, rok: 2002

A method for separation of cytokinins from auxin and abscisic acid, which allows further separation ofcylokinin ribotides from cytokinin bases, ribosidcs und glucosides and their purification on a single Oasis MCX column was developed. Due to the mixed reversed-phase and cation-exchange mode of the Oasis MCX sorbent the cationie cylokinin bases, ribosides and glucosides as well as the anionic auxin, abscisic acid and cytokinin ribotides are retained and can be sequentially eluled by solvents containing different concentrations of methanol and ammonium hydroxide. Characteristics of (he method are high recoveries of analyzed phytohormones and their sufficient purity for quantification by HPLC-ELISA (R1A) or I1PLC-MS.
Trvalý link: http://hdl.handle.net/11104/0069262