Quantitative Analysis of Ingenol in Euphorbia species via Validated Isotope Dilution Ultra-high Performance Liquid Chromatography Tandem Mass Spectrometry

0488656 - ÚEB 2019 RIV US eng J - Článek v odborném periodiku
Béres, T. - Dragull, K. - Pospíšil, Jiří - Tarkowská, Danuše - Dančák, M. - Bíba, Ondřej - Tarkowski, P. - Doležal, K. - Strnad, Miroslav
Quantitative Analysis of Ingenol in Euphorbia species via Validated Isotope Dilution Ultra-high Performance Liquid Chromatography Tandem Mass Spectrometry.
Phytochemical Analysis. Roč. 29, č. 1 (2018), s. 23-29. ISSN 0958-0344. E-ISSN 1099-1565
Grant CEP: GA ČR GA17-14007S; GA MŠMT(CZ) LO1204
Institucionální podpora: RVO:61389030
Klíčová slova: Euphorbia genus * ingenol * isotope-dilution method * mass spectrometry * ultra-high performance liquid chromatography
Obor OECD: Analytical chemistry
Impakt faktor: 1.963, rok: 2018

Introduction: Various species of the Euphorbia genus contain diterpene ingenol and ingenol mebutate (ingenol-3-angelate), a substance found in the sap of the plant Euphorbia peplus and an inducer of cell death. A gel formulation of the drug has been approved by the US Food and Drug Administration (FDA) and the European Medicines Agency (EMA) for the topical treatment of actinic keratosis. Objective: To develop a rapid and reliable method for quantification of ingenol in various plant extracts. Methodology: Methanolic extracts of 38 species of the Euphorbia genus were analysed via ultra-high performance liquid chromatography with tandem mass spectrometry (UHPLC–MS/MS) after methanolysis and solid-phase extraction (SPE) purification. The 18 O-labelled ingenol analogue was prepared and used as an internal standard for ingenol content determination and method validation. Results: The highest ingenol concentration (547 mg/kg of dry weight) was found in the lower leafless stems of E. myrsinites. The screening confirms a substantial amount of ingenol in species studied previously and furthermore, reveals some new promising candidates. Conclusion: The newly established UHPLC–MS/MS method shows to be an appropriate tool for screening of the Euphorbia genus for ingenol content and allows selection of species suitable for raw material production and/or in vitro culture initiation.
Trvalý link: http://hdl.handle.net/11104/0283205