0619372 - FGÚ 2026 RIV BA eng J - Článek v odborném periodiku
Pollak, N. - Ugwoke, Ch. K. - Umek, N. - Alibegović, A. - Janáček, Jiří - Radochová, Barbora - Cvetko, E.
Fiber-type composition and 3D capillary analysis of the human splenius capitis muscle.
Biomolecules and Biomedicine. Roč. 25, č. 6 (2025), s. 1351-1361. ISSN 2831-0896. E-ISSN 2831-090X
Grant CEP: GA ČR(CZ) GF22-02756K; GA MŠMT(CZ) EF18_046/0016045
Výzkumná infrastruktura: Czech-BioImaging III - 90250
Institucionální podpora: RVO:67985823
Klíčová slova: skeletal muscle * splenius capitis muscle * vastus lateralis muscle * capillary network * myosin heavy chain isoforms * MyHC * 3D image analysis * human
Obor OECD: Anatomy and morphology (plant science to be 1.6)
Způsob publikování: Open access
Web výsledku:
https://doi.org/10.17305/bb.2024.10787DOI: https://doi.org/10.17305/bb.2024.10787

Despite the significance of neck muscles in musculoskeletal disorders, their microscopic anatomy remains poorly characterized. This study examined the splenius capitis muscle, focusing on its fiber-type composition, fiber size, and capillary network characteristics. For comparison and validation, the vastus lateralis muscle was also analyzed. Muscle samples from 13 young male subjects (mean age ± SD: 35.7 ± 8.6 years) were collected within 24-h post-mortem during autopsy. Myosin heavy chain (MyHC) isoform expression was characterized immunohistochemically in 10 μm sections, while the capillary network architecture was assessed in 100 μm sections. Immunofluorescence staining, confocal microscopy, and 3D image analysis were employed to quantify capillary tortuosity, anisotropy, branch density (Br dens), and the length of capillaries per muscle volume (LV), per muscle fiber length (LL), per fiber surface area (LS), and per fiber volume (LVf). Compared to the vastus lateralis muscle, the splenius capitis muscle had a higher percentage of type 1 fibers (51.2% vs 39.7%), fewer type 2a fibers (16.2% vs 31.4%), and smaller fiber diameters (35.5–40.9 μm vs 47–56.1 μm). It also displayed lower Br dens (P = 0.0069), higher anisotropy (P = 0.0004), and lower LL (P < 0.0001) but higher LVf (P = 0.0486). In the splenius capitis muscle, body mass index (BMI) negatively correlated with LV (P = 0.0155), LS (P = 0.0091), LVf (P = 0.0137), and anisotropy (P = 0.0425), and positively correlated with tortuosity (P = 0.0473), indicating a reduction in the capillary network. In the vastus lateralis muscle, only LV (P = 0.0161) decreased with high BMI. This study characterized the fiber-type composition, fiber size, and 3D capillary network of the splenius capitis muscle, establishing a baseline for investigations into pathological muscle alterations.
Trvalý link: https://hdl.handle.net/11104/0366110