0604266 - ÚSMH 2025 RIV CH eng C - Konferenční příspěvek (zahraniční konf.)
Matějka, R. - Kaňoková, D. - Šupová, Monika - Matějková, J. - Chlupáč, J.
Vascular Grafts Derived from Decellularized Tissues Modified with 3D Bioprinting.
Advances in digital health and medical bioengineering, VOL 2, EHB-2023. Vol. 110. Cham: Springer, 2024, s. 406-413. ISBN 978-3-031-62520-6. ISSN 1680-0737.
[International Conference on E-Health and Bioengineering (EHB) /11./. Bucharest (RO), 09.11.2023-10.11.2023]
Grant CEP: GA MZd(CZ) NV19-02-00068
Institucionální podpora: RVO:67985891
Klíčová slova: Decellularization * 3D Bioprint * Vascular Patch * Vascular Graft
Obor OECD: Polymer science
Web výsledku:
https://doi.org/10.1007/978-3-031-62520-6_45DOI: https://doi.org/10.1007/978-3-031-62520-6_45

Cardiovascular surgery lacks suitable biomaterials for bypass surgery and patch repair. Currently available grafts for bypass surgery possess many shortcomings. In this study we are aiming to prepare vascular grafts and patches based on decellularized and lyophilized matrices (pericardium and vessels) with further recolonization with adipose derived stromal or Wharton's jelly stromal cells. Decellularization was held in a custom-built automated system allowing cyclic change of decellularization agents and cleaning water, maintaining a reproducible process. To ensure homogenous recolonization of prepared substrates the 3D bioprinting and microextrusion method was utilized. Planar tissues were recolonized using 3D bioprinting methods. Porcine collagen with incorporated cells was used. Then to coat the inner lumen of tubular tissue microextrusion method was created. It uses special microcannulas where collagen with cells was extruded, leaving inner lumen homogeneously coated. Prepared planar patches and tubular grafts were then implanted into pigs for one month observation.
Trvalý link: https://hdl.handle.net/11104/0361791