0602190 - FGÚ 2025 RIV NL eng J - Článek v odborném periodiku
Sivčev, S. - Constantin, S. - Smiljanic, K. - Sokanovic, S. J. - Fletcher, P. A. - Sherman, A. S. - Zemková, Hana - Stojilkovic, S. S.
Distribution and calcium signaling function of somatostatin receptor subtypes in rat pituitary.
Cell Calcium. Roč. 124, Dec. (2024), č. článku 102967. ISSN 0143-4160. E-ISSN 1532-1991
Institucionální podpora: RVO:67985823
Klíčová slova: somatostatin receptors * pituitary * gonadotrophs * thyrotrophs * lactotrophs * somatotrophs * voltage-gated calcium influx
Obor OECD: Neurosciences (including psychophysiology
Impakt faktor: 4.3, rok: 2023 ; AIS: 1.208, rok: 2023
Způsob publikování: Omezený přístup
Web výsledku:
https://doi.org/10.1016/j.ceca.2024.102967DOI: https://doi.org/10.1016/j.ceca.2024.102967

The somatostatin (SST) receptor family controls pituitary hormone secretion, but the distribution and specific roles of these receptors on the excitability and voltage-gated calcium signaling of hormone producing pituitary cells have not been fully characterized. Here we show that the rat pituitary gland expressed Sstr1, Sstr2, Sstr3, and Sstr5 receptor genes in a cell type-specific manner: Sstr1 and Sstr2 in thyrotrophs, Sstr3 in gonadotrophs and lactotrophs, Sstr2, Sstr3, and Sstr5 in somatotrophs, and none in corticotrophs and melanotrophs. Most gonadotrophs and thyrotrophs spontaneously fired high-amplitude single action potentials, which were silenced by SST without affecting intracellular calcium concentrations. In contrast, lactotrophs and somatotrophs spontaneously fired low-amplitude plateau-bursting action potentials in conjunction with calcium transients, both of which were silenced by SST. Moreover, SST inhibited GPCR-induced voltage-gated calcium signaling and hormone secretion in all cell types expressing SST receptors, but the inhibition was more pronounced in somatotrophs. The pattern of inhibition of electrical activity and calcium signaling was consistent with both direct and indirect inhibition of voltage-gated calcium channels, the latter being driven by cell type-specific hyperpolarization. These results indicate that the action of SST in somatotrophs is enhanced by the expression of several types of SST receptors and their slow desensitization, that SST may play a role in the electrical resynchronization of gonadotrophs, thyrotrophs, and lactotrophs, and that the lack of SST receptors in corticotrophs and melanotrophs keeps them excitable and ready to responses to stress.
Trvalý link: https://hdl.handle.net/11104/0359402