Dual effect of anandamide on spinal nociceptive transmission in control and inflammatory conditions

0583846 - FGÚ 2025 RIV NL eng J - Článek v odborném periodiku
Pontearso, Monica - Slepička, Jakub - Bhattacharyya, Anirban - Špicarová, Diana - Paleček, Jiří
Dual effect of anandamide on spinal nociceptive transmission in control and inflammatory conditions.
Biomedicine & Pharmacotherapy. Roč. 173, April (2024), č. článku 116369. ISSN 0753-3322. E-ISSN 1950-6007
Grant CEP: GA ČR GA21-02371S; GA ČR GA18-09853S; GA MŠMT(CZ) LX22NPO5104
Institucionální podpora: RVO:67985823
Klíčová slova: anandamide * CB1 * TRPV1 * FAAH * spinal cord * synaptic transmission
Obor OECD: Neurosciences (including psychophysiology
Impakt faktor: 7.5, rok: 2022
Způsob publikování: Open access
https://doi.org/10.1016/j.biopha.2024.116369

Anandamide (AEA) is an important modulator of nociception in the spinal dorsal horn, acting presynaptically through Cannabinoid (CB1) and Transient receptor potential vanilloid (TRPV1) receptors. The role of AEA (1 µM, 10 µM, and 30 µM) application on the modulation of nociceptive synaptic transmission under control and inflammatory conditions was studied by recording miniature excitatory postsynaptic currents (mEPSCs) from neurons in spinal cord slices. Inhibition of the CB1 receptors by PF514273, TRPV1 by SB366791, and the fatty acid amide hydrolase (FAAH) by URB597 was used. Under naïve conditions, the AEA application did not affect the mEPSCs frequency (1.43±0.12 Hz) when all the recorded neurons were considered. The mEPSC frequency increased (180.0±39.2%) only when AEA (30 µM) was applied with PF514273 and URB597. Analysis showed that one sub-population of neurons had synaptic input inhibited (39.1% of neurons), the second excited (43.5%), whereas 8.7% showed a mixed effect and 8.7% did not respond to the AEA. With inflammation, the AEA effect was highly inhibitory (72.7%), while the excitation was negligible (9.1%), and 18.2% were not modulated. After inflammation, more neurons (45.0%) responded even to low AEA by mEPSC frequency increase with PF514273/URB597 present. AEA-induced dual (excitatory/inhibitory) effects at the 1st nociceptive synapse should be considered when developing analgesics targeting the endocannabinoid system. These findings contrast the clear inhibitory effects of the AEA precursor 20:4-NAPE application described previously and suggest that modulation of endogenous AEA production may be more favorable for analgesic treatments.
Trvalý link: https://hdl.handle.net/11104/0351849