Disordered-to-ordered transitions in assembly factors allow the complex II catalytic subunit to switch binding partners

0582380 - BTÚ 2025 US eng J - Článek v odborném periodiku
Sharma, P. - Maklashina, E. - Voehler, M. - Balintová, Soňa - Dvořáková, Šárka - Kraus, Michal - Váňová Hadrava, Kateřina - Nahácka, Zuzana - Zobalová, Renata - Boukalová, Štěpána - Čunátová, Kristýna - Mráček, Tomáš - Ghayee, H. K. - Pacak, K. - Rohlena, Jakub - Neužil, Jiří - Cecchini, G. - Iverson, T. M.
Disordered-to-ordered transitions in assembly factors allow the complex II catalytic subunit to switch binding partners.
Nature Communications. Roč. 15, č. 1 (2024), č. článku 473. E-ISSN 2041-1723
Grant CEP: GA MZd(CZ) NU23-03-00226; GA ČR(CZ) GA23-05303S; GA ČR(CZ) GJ20-11724Y; GA ČR GA19-20553S; GA ČR(CZ) GX21-04607X; GA ČR(CZ) GA20-05942S; GA ČR(CZ) GA20-25768S
Institucionální podpora: RVO:86652036
Klíčová slova: onset optic atrophy * succinate-dehydrogenase * crystal-structure * structural basis * chemical-shifts
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 16.6, rok: 2022
Způsob publikování: Open access
https://www.nature.com/articles/s41467-023-44563-7

Complex II (CII) activity controls phenomena that require crosstalk between metabolism and signaling, including neurodegeneration, cancer metabolism, immune activation, and ischemia-reperfusion injury. CII activity can be regulated at the level of assembly, a process that leverages metastable assembly intermediates. The nature of these intermediates and how CII subunits transfer between metastable complexes remains unclear. In this work, we identify metastable species containing the SDHA subunit and its assembly factors, and we assign a preferred temporal sequence of appearance of these species during CII assembly. Structures of two species show that the assembly factors undergo disordered-to-ordered transitions without the appearance of significant secondary structure. The findings identify that intrinsically disordered regions are critical in regulating CII assembly, an observation that has implications for the control of assembly in other biomolecular complexes.
Trvalý link: https://hdl.handle.net/11104/0353431