Počet záznamů: 1
Impairment of adrenergically-regulated thermogenesis in brown fat of obesity-resistant mice is compensated by non-shivering thermogenesis in skeletal muscle
- 1.0570490 - FGÚ 2024 RIV NL eng J - Článek v odborném periodiku
Janovská, Petra - Zouhar, Petr - Bardová, Kristina - Otáhal, Jakub - Vrbacký, Marek - Mráček, Tomáš - Adamcová, Kateřina - Leňková, Lucie - Funda, Jiří - Čajka, Tomáš - Drahota, Zdeněk - Stanić, Sara - Rustan, A. C. - Horáková, Olga - Houštěk, Josef - Rossmeisl, Martin - Kopecký, Jan
Impairment of adrenergically-regulated thermogenesis in brown fat of obesity-resistant mice is compensated by non-shivering thermogenesis in skeletal muscle.
Molecular Metabolism. Roč. 69, March (2023), č. článku 101683. ISSN 2212-8778. E-ISSN 2212-8778
Grant CEP: GA MŠk(CZ) LX22NPO5104; GA ČR(CZ) GA18-04483S; GA ČR(CZ) GA19-02411S; GA ČR(CZ) GA21-18993S; GA ČR(CZ) GA22-07004S; GA MŠk(CZ) LM2018129
Institucionální podpora: RVO:67985823
Klíčová slova: non-shivering thermogenesis * sarcolipin * mitochondrial supercomplex * skeletal muscle * brown adipose tissue * obesity
Obor OECD: Endocrinology and metabolism (including diabetes, hormones)
Impakt faktor: 8.100, rok: 2022
Způsob publikování: Open access
Objective:Non-shivering thermogenesis (NST) mediated by uncoupling protein 1 (UCP1) in brown adipose tissue (BAT) can be activated via the adrenergic system in response to cold or diet, contributing to both thermal and energy homeostasis. Other mechanisms, including metabolism of skeletal muscle, may also be involved in NST. However, relative contribution of these energy dissipating pathways and their adaptability remain a matter of long-standing controversy.Methods:We used warm-acclimated (30 °C) mice to characterize the effect of an up to 7-day cold acclimation (6 °C, CA) on thermoregulatory thermogenesis, comparing inbred mice with a genetic background conferring resistance (A/J) or susceptibility (C57BL/6 J) to obesity.Results:Both warm-acclimated C57BL/6 J and A/J mice exhibited similar cold endurance, assessed as a capability to maintain core body temperature during acute exposure to cold, which improved in response to CA, resulting in comparable cold endurance and similar induction of UCP1 protein in BAT of mice of both genotypes. Despite this, adrenergic NST in BAT was induced only in C57BL/6 J, not in A/J mice subjected to CA. Cold tolerance phenotype of A/J mice subjected to CA was not based on increased shivering, improved insulation, or changes in physical activity. On the contrary, lipidomic, proteomic and gene expression analyses along with palmitoyl carnitine oxidation and cytochrome c oxidase activity revealed induction of lipid oxidation exclusively in skeletal muscle of A/J mice subjected to CA. These changes appear to be related to skeletal muscle NST, mediated by sarcolipin-induced uncoupling of sarco(endo)plasmic reticulum calcium ATPase pump activity and accentuated by changes in mitochondrial respiratory chain supercomplexes assembly.Conclusions:Our results suggest that NST in skeletal muscle could be adaptively augmented in the face of insufficient adrenergic NST in BAT, depending on the genetic background of the mice. It may provide both protection from cold and resistance to obesity, more effectively than BAT.
Trvalý link: https://hdl.handle.net/11104/0341794
Počet záznamů: 1