Expression of Selected miRNAs in Normal and Cancer-Associated Fibroblasts and in BxPc3 and MIA PaCa-2 Cell Lines of Pancreatic Ductal Adenocarcinoma

0570268 - ÚMG 2024 RIV CH eng J - Článek v odborném periodiku
Mandys, V. - Popov, A. - Gürlich, R. - Havránek, Jan - Pfeiferová, Lucie - Kolář, Michal - Vránová, J. - Smetana Jr, K. - Lacina, L. - Szabo, P.
Expression of Selected miRNAs in Normal and Cancer-Associated Fibroblasts and in BxPc3 and MIA PaCa-2 Cell Lines of Pancreatic Ductal Adenocarcinoma.
International Journal of Molecular Sciences. Roč. 24, č. 4 (2023), č. článku 3617. E-ISSN 1422-0067
Grant CEP: GA MŠMT LX22NPO5102; GA MŠMT(CZ) EF16_019/0000785
Institucionální podpora: RVO:68378050
Klíčová slova: pancreas * cancer-associated fibroblast * miRNA * miR-21 * miR-210 * il-6 * hypoxia
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 5.6, rok: 2022
Způsob publikování: Open access
https://www.mdpi.com/1422-0067/24/4/3617

Therapy for pancreatic ductal adenocarcinoma remains challenging, and the chances of a complete cure are very limited. As in other types of cancer, the expression and role of miRNAs in controlling the biological properties of this type of tumor have been extensively studied. A better insight into miRNA biology seems critical to refining diagnostics and improving their therapeutic potential. In this study, we focused on the expression of miR-21,96,196a,210, and217 in normal fibroblasts, cancer-associated fibroblasts prepared from a ductal adenocarcinoma of the pancreas, and pancreatic carcinoma cell lines. We compared these data with miRNAs in homogenates of paraffin-embedded sections from normal pancreatic tissues. In cancer-associated fibroblasts and cancer cell lines, miRNAs differed significantly from the normal tissue. In detail, miR-21 and210 were significantly upregulated, while miR-217 was downregulated. Similar transcription profiles were earlier reported in cancer-associated fibroblasts exposed to hypoxia. However, the cells in our study were cultured under normoxic conditions. We also noted a relation to IL-6 production. In conclusion, cultured cancer-associated fibroblasts and carcinoma cells reflect miR-21 and210 expression similarly to the cancer tissue samples harvested from the patients.
Trvalý link: https://hdl.handle.net/11104/0341706