The Tale-Tell Heart: Evolutionary tetrapod shift from aquatic to terrestrial life-style reflected in heart changes in axolotl (Ambystoma mexicanum)

0558188 - FGÚ 2023 RIV US eng J - Článek v odborném periodiku
Olejníčková, Veronika - Kolesová, Hana - Bartoš, M. - Sedmera, David - Gregorovičová, Martina
The Tale-Tell Heart: Evolutionary tetrapod shift from aquatic to terrestrial life-style reflected in heart changes in axolotl (Ambystoma mexicanum).
Developmental Dynamics. Roč. 251, č. 6 (2022), s. 1004-1014. ISSN 1058-8388. E-ISSN 1097-0177
Institucionální podpora: RVO:67985823
Klíčová slova: atrial septation * metamorphosis * micro-CT * optical mapping * trabeculae * ventricular septation
Obor OECD: Developmental biology
Impakt faktor: 2.500, rok: 2022
Způsob publikování: Omezený přístup
https://doi.org/10.1002/dvdy.413

Background During amphibian metamorphosis, the crucial moment lies in the rearrangement of the heart, reflecting the changes in circulatory demands. However, little is known about the exact shifts linked with this rearrangement. Here, we demonstrate such myocardial changes in axolotl (Ambystoma mexicanum) from the morphological and physiological point of view. Results Micro-CT and histological analysis showed changes in ventricular trabeculae organization, completion of the atrial septum and its connection to the atrioventricular valve. Based on Myosin Heavy Chain and Smooth Muscle Actin expression we distinguished metamorphosis-induced changes in myocardial differentiation at the ventricular trabeculae and atrioventricular canal. Using optical mapping, faster speed of conduction through the atrioventricular canal was demonstrated in metamorphic animals. No differences between the groups were observed in the heart rates, ventricular activation times, and activation patterns. Conclusions Transition from aquatic to terrestrial life-style is reflected in the heart morphology and function. Rebuilding of the axolotl heart during metamorphosis was connected with reorganization of ventricular trabeculae, completion of the atrial septum and its connection to the atrioventricular valve, and acceleration of AV conduction.
Trvalý link: http://hdl.handle.net/11104/0332202