Počet záznamů: 1  

Rabbit Endothelial Progenitor Cells Derived From Peripheral Blood and Bone Marrow: An Ultrastructural Comparative Study

  1. 1.
    0558119 - FGÚ 2023 RIV US eng J - Článek v odborném periodiku
    Ďúranová, H. - Valková, V. - Olexiková, L. - Radochová, Barbora - Balazi, A. - Chrenek, P. - Vašíček, J.
    Rabbit Endothelial Progenitor Cells Derived From Peripheral Blood and Bone Marrow: An Ultrastructural Comparative Study.
    Microscopy and Microanalysis. Roč. 28, č. 3 (2022), s. 756-766. ISSN 1431-9276. E-ISSN 1435-8115
    Institucionální podpora: RVO:67985823
    Klíčová slova: bone marrow * endothelial progenitor cells * peripheral blood * stereological approaches * transmission electron microscopy
    Obor OECD: Cell biology
    Impakt faktor: 2.8, rok: 2022
    Způsob publikování: Omezený přístup
    https://doi.org/10.1017/S143192762200037X

    The present study was designed to compare the ultrastructure of early endothelial progenitor cells (EPCs) derived from rabbit peripheral blood (PB-EPCs) and bone marrow (BM-EPCs). After the cells had been isolated and cultivated up to passage 3, microphotographs obtained from transmission electron microscope were evaluated from qualitative and quantitative (unbiased stereological approaches) points of view. Our results revealed that both cell populations displayed almost identical ultrastructural characteristics represented by abundant cellular organelles dispersed in the cytoplasm. Moreover, the presence of very occasionally occurring mature endothelial-specific Weibel-Palade bodies (WPBs) confirmed their endothelial lineage origin. The more advanced stage of their differentiation was also demonstrated by the relatively low nucleus/cytoplasm (N/C) ratios (0.41 +/- 0.19 in PB-EPCs, 0.37 +/- 0.25 in BM-EPCs). Between PB-EPCs and BM-EPCs, no differences in proportions of cells occupied by nucleus (28.13 +/- 8.97 versus 25.10 +/- 11.48%), mitochondria (3.71 +/- 1.33 versus 4.23 +/- 1.00%), and lipid droplets (0.65 +/- 1.01 versus 0.36 +/- 0.40%), as well as in estimations of the organelles surface densities were found. The data provide the first quantitative evaluation of the organelles of interest in PB-EPCs and BM-EPCs, and they can serve as a research framework for understanding cellular function.
    Trvalý link: http://hdl.handle.net/11104/0331924

     
     
Počet záznamů: 1  

  Tyto stránky využívají soubory cookies, které usnadňují jejich prohlížení. Další informace o tom jak používáme cookies.