CDC25B is required for the metaphase I-metaphase II transition in mouse oocytes

0557508 - ÚŽFG 2023 RIV GB eng J - Článek v odborném periodiku
Ferencová, Ivana - Vaškovičová, Michaela - Drutovič, Dávid - Knoblochová, Lucie - Macůrek, Libor - Schultz, R. M. - Šolc, Petr
CDC25B is required for the metaphase I-metaphase II transition in mouse oocytes.
Journal of Cell Science. Roč. 135, č. 6 (2022), č. článku jcs252924. ISSN 0021-9533. E-ISSN 1477-9137
Grant CEP: GA MŠMT(CZ) LTAUSA17097; GA ČR GA17-04742S
Institucionální podpora: RVO:67985904 ; RVO:68378050
Klíčová slova: resumption of meiosis * meiotic maturation * mouse oocytes
Obor OECD: Developmental biology
Impakt faktor: 4, rok: 2022
Způsob publikování: Open access
https://journals.biologists.com/jcs/article-abstract/135/6/jcs252924/274776/CDC25B-is-required-for-the-metaphase-I-metaphase?redirectedFrom=fulltext

Mammalian oocytes are arrested at meiotic prophase I. The dual-specificity phosphatase CDC25B is essential for cyclin-dependent kinase 1 (CDK1) activation that drives resumption of meiosis. CDC25B reverses the inhibitory effect of the protein kinases WEE1 and MYT1 on CDK1 activation. Cdc25b(-/-) female mice are infertile because oocytes cannot activate CDK1. To identify a role for CDC25B following resumption of meiosis, we restored CDK1 activation in Cdc25b(-/-) oocytes by inhibiting WEE1 and MYT1, or expressing EGFP-CDC25A or constitutively active EGFP-CDK1 from microinjected complementary RNAs. Forced CDK1 activation in Cdc25b(-/- )oocytes allowed resumption of meiosis, but oocytes mostly arrested at metaphase I (MI) with intact spindles. Similarly, approximately a third of Cdc25b(-/-)( )oocytes with a reduced amount of CDC25B arrested in MI. MI-arrested Cdc25b(-/-) oocytes also displayed a transient decrease in CDK1 activity similar to Cdc25b(+/-) oocytes during the MI-MII transition, whereas Cdc25b(+/+ )oocytes exhibited only a partial anaphase-promoting complex/cyclosome activation and anaphase I entry. Thus, CDC25B is necessary for the resumption of meiosis and the MI-MII transition.
Trvalý link: http://hdl.handle.net/11104/0331472