Počet záznamů: 1
Time-Dependent Changes in Protein Composition of Medial Prefrontal Cortex in Rats with Neuropathic Pain
- 1.0555833 - FGÚ 2023 RIV CH eng J - Článek v odborném periodiku
Ujčíková, Hana - Robles, D. - Yue, X. - Svoboda, Petr - Lee, Y. S. - Navratilova, E.
Time-Dependent Changes in Protein Composition of Medial Prefrontal Cortex in Rats with Neuropathic Pain.
International Journal of Molecular Sciences. Roč. 23, č. 2 (2022), č. článku 955. E-ISSN 1422-0067
Institucionální podpora: RVO:67985823
Klíčová slova: neuropathic pain * affective dimension of pain * pain chronification * prefrontal cortex * proteomics
Obor OECD: Physiology (including cytology)
Impakt faktor: 5.600, rok: 2022
Způsob publikování: Open access
Chronic pain is associated with time-dependent structural and functional reorganization of the prefrontal cortex that may reflect adaptive pain compensatory and/or maladaptive pain-promoting mechanisms. However, the molecular underpinnings of these changes and whether there are time-dependent relationships to pain progression are not well characterized. In this study, we analyzed protein composition in the medial prefrontal cortex (mPFC) of rats at two timepoints after spinal nerve ligation (SNL) using two-dimensional gel electrophoresis (2D-ELFO) and liquid chromatography with tandem mass spectrometry (LC-MS/MS). SNL, but not sham-operated, rats developed persistent tactile allodynia and thermal hyperalgesia, confirming the presence of experimental neuropathic pain. Two weeks after SNL (early timepoint), we identified 11 proteins involved in signal transduction, protein transport, cell homeostasis, metabolism, and apoptosis, as well as heat-shock proteins and chaperones that were upregulated by more than 1.5-fold compared to the sham-operated rats. Interestingly, there were only four significantly altered proteins identified at 8 weeks after SNL (late timepoint). These findings demonstrate extensive time-dependent modifications of protein expression in the rat mPFC under a chronic neuropathic pain state that might underlie the evolution of chronic pain characterized by early pain-compensatory and later aberrant mechanisms.
Trvalý link: http://hdl.handle.net/11104/0330300
Počet záznamů: 1