Healing and Angiogenic Properties of Collagen/Chitosan Scaffolds Enriched with Hyperstable FGF2-STAB(R) Protein: In Vitro, Ex Ovo and In Vivo Comprehensive Evaluation

0553280 - ÚEM 2022 RIV CH eng J - Článek v odborném periodiku
Vojtová, L. - Pavliňáková, V. - Muchova, J. - Kacvinská, K. - Brtníková, J. - Knoz, M. - Lipový, B. - Faldyna, M. - Gopfert, E. - Holoubek, J. - Pavlovský, Z. - Vicenová, M. - Hefka Blahnová, Veronika - Hearnden, V. - Filová, Eva
Healing and Angiogenic Properties of Collagen/Chitosan Scaffolds Enriched with Hyperstable FGF2-STAB(R) Protein: In Vitro, Ex Ovo and In Vivo Comprehensive Evaluation.
Biomedicines. Roč. 9, č. 6 (2021), č. článku 590. E-ISSN 2227-9059
Grant CEP: GA MŠMT(CZ) LO1508
Institucionální podpora: RVO:68378041
Klíčová slova: collagen * chitosan * scaffold * FGF2 * skin regeneration * tissue engineering
Obor OECD: Biomaterials (as related to medical implants, devices, sensors)
Impakt faktor: 4.757, rok: 2021
Způsob publikování: Open access
https://www.mdpi.com/2227-9059/9/6/590

Wound healing is a process regulated by a complex interaction of multiple growth factors including fibroblast growth factor 2 (FGF2). Although FGF2 appears in several tissue engineered studies, its applications are limited due to its low stability both in vitro and in vivo. Here, this shortcoming is overcome by a unique nine-point mutant of the low molecular weight isoform FGF2 retaining full biological activity even after twenty days at 37 degrees C. Crosslinked freeze-dried 3D porous collagen/chitosan scaffolds enriched with this hyper stable recombinant human protein named FGF2-STAB(R) were tested for in vitro biocompatibility and cytotoxicity using murine 3T3-A31 fibroblasts, for angiogenic potential using an ex ovo chick chorioallantoic membrane assay and for wound healing in vivo with 3-month old white New Zealand rabbits. Metabolic activity assays indicated the positive effect of FGF2-STAB(R) already at very low concentrations (0.01 mu g/mL). The angiogenic properties examined ex ovo showed enhanced vascularization of the tested scaffolds. Histological evaluation and gene expression analysis by RT-qPCR proved newly formed granulation tissue at the place of a previous skin defect without significant inflammation infiltration in vivo. This work highlights the safety and biocompatibility of newly developed crosslinked collagen/chitosan scaffolds involving FGF2-STAB(R) protein. Moreover, these sponges could be used as scaffolds for growing cells for dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration.
Trvalý link: http://hdl.handle.net/11104/0328263