Construction and application of the amperometric uric acid biosensors based on the covalent immobilization of uricase by different strategies

0546183 - ÚFCH JH 2022 RIV CZ eng C - Konferenční příspěvek (zahraniční konf.)
Tvorynska, Sofiia - Barek, J. - Josypčuk, Bohdan
Construction and application of the amperometric uric acid biosensors based on the covalent immobilization of uricase by different strategies.
Proceedings of the 17th ISC Modern Analytical Chemistry. Prague: Charles University, 2021 - (Nesměrák, K.), s. 145-151. ISBN 978-80-7444-089-2.
[ISC Modern Analytical Chemistry /17./. Prague (CZ), 16.09.2021-17.09.2021]
Grant CEP: GA ČR(CZ) GA20-01589S
Institucionální podpora: RVO:61388955
Klíčová slova: amalgam electrode * amperometric biosensor * uric acid
Obor OECD: Physical chemistry

In this work, a promising combination of a biosensor based on the
enzymatic mini-reactor with the detection principle of four-electron
reduction of the consumed oxygen at highly negative potential has
been developed for uric acid determination using flow injection
analysis. The construction of the biosensor provides a spatial
segregation of the biorecognition (uricase-based mini-reactor) and
detection (tubular detector of silver solid amalgam (TD-p-AgSA))
parts. To find out the most appropriate enzyme immobilization
protocol, three different strategies of the covalent attachment for
uricase from Bacillus fastidiosus have been compared. It was found
that the biosensor with the mini-reactor based on the covalent
attachment of uricase via glutaraldehyde to -NH2 functionalized
mesoporous silica powder MCM-41 showed extremely high stability
(>1 year) and reusability (at least 600 measurements) The biosensor's
practical applicability was confirmed by successful determination
of uric acid in human urine.
Trvalý link: http://hdl.handle.net/11104/0322725