The Photosystem II Assembly Factor Ycf48 from the Cyanobacterium Synechocystis sp. PCC 6803 Is Lipidated Using an Atypical Lipobox Sequence

0542484 - MBÚ 2022 RIV CH eng J - Článek v odborném periodiku
Knoppová, Jana - Yu, J. - Janouškovec, Jan - Halada, Petr - Nixon, P. J. - Whitelegge, J. P. - Komenda, Josef
The Photosystem II Assembly Factor Ycf48 from the Cyanobacterium Synechocystis sp. PCC 6803 Is Lipidated Using an Atypical Lipobox Sequence.
International Journal of Molecular Sciences. Roč. 22, č. 7 (2021), č. článku 3733. E-ISSN 1422-0067
Grant CEP: GA ČR(CZ) GX19-29225X
GRANT EU: European Commission(CZ) 854126 - PhotoRedesign
Institucionální podpora: RVO:61388971
Klíčová slova: photosystem II * photosynthesis * chlorophyll-binding proteins
Obor OECD: Microbiology
Impakt faktor: 6.208, rok: 2021
Způsob publikování: Open access
https://www.mdpi.com/1422-0067/22/7/3733

Photochemical energy conversion during oxygenic photosynthesis is performed by membrane-embedded chlorophyll-binding protein complexes. The biogenesis and maintenance of these complexes requires auxiliary protein factors that optimize the assembly process and protect nascent complexes from photodamage. In cyanobacteria, several lipoproteins contribute to the biogenesis and function of the photosystem II (PSII) complex. They include CyanoP, CyanoQ, and Psb27, which are all attached to the lumenal side of PSII complexes. Here, we show that the lumenal Ycf48 assembly factor found in the cyanobacterium Synechocystis sp. PCC 6803 is also a lipoprotein. Detailed mass spectrometric analysis of the isolated protein supported by site-directed mutagenesis experiments indicates lipidation of the N-terminal C29 residue of Ycf48 and removal of three amino acids from the C-terminus. The lipobox sequence in Ycf48 contains a cysteine residue at the3 position compared to Leu/Val/Ile residues found in the canonical lipobox sequence. The atypical Ycf48 lipobox sequence is present in most cyanobacteria but is absent in eukaryotes. A possible role for lipoproteins in the coordinated assembly of cyanobacterial PSII is discussed.
Trvalý link: http://hdl.handle.net/11104/0319887