Počet záznamů: 1  

Dysregulation of endocannabinoid concentrations in human subcutaneous adipose tissue in obesity and modulation by omega-3 polyunsaturated fatty acids

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    0541699 - FGÚ 2022 RIV GB eng J - Článek v odborném periodiku
    Fisk, H. L. - Childs, C. E. - Miles, E. A. - Ayres, R. - Noakes, P. S. - Paras-Chavez, C. - Kuda, Ondřej - Kopecký, Jan - Antoun, E. - Lillycrop, K. A. - Calder, P. C.
    Dysregulation of endocannabinoid concentrations in human subcutaneous adipose tissue in obesity and modulation by omega-3 polyunsaturated fatty acids.
    Clinical science. Roč. 135, č. 1 (2021), s. 185-200. ISSN 0143-5221. E-ISSN 1470-8736
    Institucionální podpora: RVO:67985823
    Klíčová slova: adipose tissue * endocannabinoids * LC n-3 PUFA * lipids * obesity
    Obor OECD: Endocrinology and metabolism (including diabetes, hormones)
    Impakt faktor: 6.876, rok: 2021
    Způsob publikování: Omezený přístup
    https://doi.org/10.1042/CS20201060

    Obesity is believed to be associated with a dysregulated endocannabinoid system which may reflect enhanced inflammation. However, reports of this in human white adipose tissue (WAT) are limited and inconclusive. Marine long-chain omega-3 polyunsaturated fatty acids (LC n-3 PUFAs) have anti-inflammatory actions and therefore may improve obesity-associated adipose tissue inflammation. Therefore, fatty acid (FA) concentrations, endocannabinoid concentrations, and gene expression were assessed in subcutaneous WAT (scWAT) biopsies from healthy normal weight individuals (BMI 18.5–25 kg/m2) and individuals living with metabolically healthy obesity (BMI 30–40 kg/m2) prior to and following a 12-week intervention with 3 g fish oil/day (1.1 g eicosapentaenoic acid (EPA) + 0.8 g DHA) or 3 g corn oil/day (placebo). WAT from individuals living with metabolically healthy obesity had higher n-6 PUFAs and EPA, higher concentrations of two endocannabinoids (anandamide (AEA) and eicosapentaenoyl ethanolamide (EPEA)), higher expression of phospholipase A2 Group IID (PLA2G2D) and phospholipase A2 Group IVA (PLA2G4A), and lower expression of CNR1. In response to fish oil intervention, WAT EPA increased to a similar extent in both BMI groups, and WAT DHA increased by a greater extent in normal weight individuals. WAT EPEA and docosahexaenoyl ethanolamide (DHEA) increased in normal weight individuals only and WAT 2-arachidonyl glycerol (2-AG) decreased in individuals living with metabolically healthy obesity only. Altered WAT fatty acid, endocannabinoid, and gene expression profiles in metabolically healthy obesity at baseline may be linked. WAT incorporates n-3 PUFAs when their intake is increased which affects the endocannabinoid system, however, effects appear greater in normal weight individuals than in those living with metabolically healthy obesity.
    Trvalý link: http://hdl.handle.net/11104/0319229

     
     
Počet záznamů: 1