Počet záznamů: 1
Human osteoblast-like SAOS-2 cells on submicron-scale fibers coated with nanocrystalline diamond films
- 1.0541653 - FGÚ 2022 RIV NL eng J - Článek v odborném periodiku
Steinerová, Marie - Matějka, Roman - Štěpanovská, Jana - Filová, Elena - Staňková, Ľubica - Rysová, M. - Martinová, L. - Dragounová, Helena - Domonkos, Mária - Artemenko, Anna - Babchenko, Oleg - Otáhal, M. - Bačáková, Lucie - Kromka, Alexander
Human osteoblast-like SAOS-2 cells on submicron-scale fibers coated with nanocrystalline diamond films.
Materials Science & Engineering C-Materials for Biological Applications. Roč. 121, Feb (2021), č. článku 111792. ISSN 0928-4931. E-ISSN 1873-0191
Grant CEP: GA ČR(CZ) GA19-02891S; GA MZd(CZ) NV19-02-00068; GA MŠk(CZ) EF16_019/0000760; GA MŠk LM2018110
Výzkumná infrastruktura: CzechNanoLab - 90110
Institucionální podpora: RVO:67985823 ; RVO:68378271
Klíčová slova: nanocrystalline diamond * submicron fibers * hierarchical organization * silica nanostructures * osteoblast proliferation * osteogenic differentiation * collagen * alkaline phosphatase * osteocalcin
Obor OECD: Biomaterials (as related to medical implants, devices, sensors); Biophysics (FZU-D)
Impakt faktor: 8.457, rok: 2021
Způsob publikování: Omezený přístup
A unique composite nanodiamond-based porous material with a hierarchically-organized submicron-nanostructure was constructed for potential bone tissue engineering. This material consisted of submicron fibers prepared by electrospinning of silicon oxide (SiOx), which were oxygen-terminated (O-SiOx) and were hermetically coated with nanocrystalline diamond (NCD) films. The NCD films were then terminated with hydrogen (HNCD) or oxygen (O-NCD). The materials were tested as substrates for the adhesion, growth and osteogenic differentiation of human osteoblast-like Saos-2 cells. The number and the spreading area of the initially adhered cells, their growth rate during 7 days after seeding and the activity of alkaline phosphatase (ALP) were significantly higher on the NCD-coated samples than on the uncoated 0-SiO x samples. In addition, the concentration of type I collagen was significantly higher in the cells on the O-NCD-coated samples than on the bare O-SiOx samples. The observed differences could be attributed to the tunable wettability of NCD and to the more appropriate surface morphology of the NCD-coated samples in contrast to the less stable, rapidly eroding bare SiOx surface. The H-NCD coatings and the O-NCD coatings both promoted similar initial adhesion of Saos-2 cells, but the subsequent cell proliferation activity was higher on the O-NCD-coated samples. The concentration of beta-actin, vinculin, type I collagen and alkaline phosphatase (ALP), the ALP activity, and also the calcium deposition tended to be higher in the cells on the O-NCD-coated samples than on the H-NCD-coated samples, although these differences did not reach statistical significance. The improved cell performance on the O-NCD-coated samples could be attributed to higher wettability of these samples (water drop contact angle less than 10 degrees), while the H-NCD-coated samples were hydrophobic (contact angle >701. NCD-coated porous SiOx meshes can therefore be considered as appropriate scaffolds for bone tissue engineering, particularly those with an O-terminated NCD coating.
Trvalý link: http://hdl.handle.net/11104/0319188
Počet záznamů: 1