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Glucocorticoids reset circadian clock in choroid plexus via period genes

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    0541621 - FGÚ 2022 RIV GB eng J - Článek v odborném periodiku
    Liška, Karolína - Sládek, Martin - Čečmanová, Vendula - Sumová, Alena
    Glucocorticoids reset circadian clock in choroid plexus via period genes.
    Journal of Endocrinology. Roč. 248, č. 2 (2021), s. 155-166. ISSN 0022-0795. E-ISSN 1479-6805
    Grant CEP: GA MŠk(CZ) LM2015062; GA ČR(CZ) GBP304/12/G069
    Institucionální podpora: RVO:67985823
    Klíčová slova: choroid plexus * circadian clock * glucocorticoids * dexamethasone * circadian entrainment * phase-response curve
    Obor OECD: Physiology (including cytology)
    Impakt faktor: 4.286, rok: 2020
    Způsob publikování: Open access s časovým embargem
    https://doi.org/10.1530/JOE-20-0526

    The epithelial cells of choroid plexus (CP) in brain ventricles produce cerebrospinal fluid and act as the blood-cerebrospinal fluid barrier. In this study, we confirmed that CP in the 4th ventricle is composed of cellular oscillators that all harbor glucocorticoid receptors and are mutually synchronized to produce a robust clock gene expression rhythm detectable at the tissue level in vivo and in vitro. Animals lacking glucocorticoids (GCs) due to surgical removal of adrenal glands had Per1, Per2, Nr1d1 and Bmal1 clock gene rhythmicity in their CP significantly dampened, whereas subjecting them to daily bouts of synthetic GC analog, dexamethasone (DEX), reinforced those rhythms. We verified these in vivo effects using an in vitro model of organotypic CP explants, depending on the time of its application, DEX significantly increased the amplitude and efficiently reset the phase of the CP clock. The results are the first description of a PRC for a non-neuronal clock in the brain, demonstrating that CP clock shares some properties with the non-neuronal clocks elsewhere in the body. Finally, we found that DEX exhibited multiple synergic effects on the CP clock, including acute activation of Per1 expression and change of PER2 protein turnover rate. The DEX-induced shifts of the CP clock were partially mediated via PKA-ERK1/2 pathway. The results provide the first evidence that the GC rhythm strengthens and entrains the clock in the CP helping thus fine-tune the brain environment according to time of day.
    Trvalý link: http://hdl.handle.net/11104/0319154

     
     
Počet záznamů: 1