Počet záznamů: 1
Expression and distribution of CD151 as a partner of alpha6 integrin in male germ cells
- 1.0534683 - BTÚ 2021 RIV GB eng J - Článek v odborném periodiku
Jankovičová, J. - Frolíková, Michaela - Páleníková, Veronika - Valášková, Eliška - Černý, Jiří - Šecová, P. - Bartokova, M. - Horovská, L. - Maňásková-Postlerová, Pavla - Antalíková, J. - Komrsková, Kateřina
Expression and distribution of CD151 as a partner of alpha6 integrin in male germ cells.
Scientific Reports. Roč. 10, č. 1 (2020), č. článku 4374. ISSN 2045-2322. E-ISSN 2045-2322
Grant CEP: GA ČR(CZ) GA18-11275S; GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:86652036
Klíčová slova: sperm-egg fusion * tetraspanin cd151 * alpha(6)beta(4) integrin * estrogen-receptor * membrane
Obor OECD: 1.7 Other natural sciences
Impakt faktor: 4.380, rok: 2020
Způsob publikování: Open access
https://www.nature.com/articles/s41598-020-61334-2.pdf
The physiological importance of CD151 tetraspanin is known from somatic cells and its outside-in signalling through integrins was described. In male germ cells, two tetraspanins, CD9 and CD81, are involved in sperm-egg membrane fusion, and similarly to integrins, they occupy characteristic regions. We report here on a newly discovered presence of CD151 in sperm, and present its expression and distribution during spermatogenesis and sperm transition during the acrosome reaction. We traced CD151 gene and protein expression in testicular cell subpopulations, with strong enrichment in spermatogonia and spermatids. The testicular and epididymal localization pattern is designated to the sperm head primary fusion site called the equatorial segment and when compared to the acrosome vesicle status, CD151 was located into the inner acrosomal membrane overlying the nucleus. Moreover, we show CD151 interaction with alpha 6 integrin subunit, which forms a dimer with beta 4 as a part of cisprotein interactions within sperm prior to gamete fusion. We used mammalian species with distinct sperm morphology and sperm maturation such as mouse and bull and compared the results with human. In conclusion, the delivered findings characterise CD151 as a novel sperm tetraspanin network member and provide knowledge on its physiology in male germ cells.
Trvalý link: http://hdl.handle.net/11104/0313123
Počet záznamů: 1