Heat treatment dependent cytotoxicity of silicalite-1 films deposited on Ti-6Al-4V alloy evaluated by bone-derived cells

0532333 - FGÚ 2021 RIV GB eng J - Článek v odborném periodiku
Němčáková, Ivana - Jirka, Ivan - Doubková, Martina - Bačáková, Lucie
Heat treatment dependent cytotoxicity of silicalite-1 films deposited on Ti-6Al-4V alloy evaluated by bone-derived cells.
Scientific Reports. Roč. 10, č. 1 (2020), č. článku 9456. ISSN 2045-2322. E-ISSN 2045-2322
Grant CEP: GA ČR(CZ) GA16-02681S; GA ČR(CZ) GA19-02891S
Institucionální podpora: RVO:67985823 ; RVO:61388955
Klíčová slova: heat treatment * cytotoxicity * silicate-1 * DNA damage * bone-derived cells
Obor OECD: Biomaterials (as related to medical implants, devices, sensors); Physical chemistry (UFCH-W)
Impakt faktor: 4.380, rok: 2020
Způsob publikování: Open access
https://www.nature.com/articles/s41598-020-66228-x

A silicalite-1 film (SF) deposited on Ti-6Al-4V alloy was investigated in this study as a promising coating for metallic implants. Two forms of SFs were prepared: as-synthesized SFs (SF-RT), and SFs heated up to 500 degrees C (SF-500) to remove the excess of template species from the SF surface. The SFs were characterized in detail by X-ray photoelectron spectroscopy (XPS), by Fourier transform infrared spectroscopy (FTIR), by scanning electron microscopy (SEM) and water contact angle measurements (WCA). Two types of bone-derived cells (hFOB 1.19 non-tumor fetal osteoblast cell line and U-2 OS osteosarcoma cell line) were used for a biocompatibility assessment. The initial adhesion of hFOB 1.19 cells, evaluated by cell numbers and cell spreading area, was better supported by SF-500 than by SF-RT. While no increase in cell membrane damage, in ROS generation and in TNF-alpha secretion of bone-derived cells grown on both SFs was found, gamma H2AX staining revealed an elevated DNA damage response of U-2 OS cells grown on heat-treated samples (SF-500). This study also discusses differences between osteosarcoma cell lines and non-tumor osteoblastic cells, stressing the importance of choosing the right cell type model.
Trvalý link: http://hdl.handle.net/11104/0310842