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Constant-current chronopotentiometric stripping detection of bovine serum albumin on silver amalgam particles

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    0524235 - BFÚ 2021 RIV CH eng J - Článek v odborném periodiku
    Šebest, Peter - Ostatná, Veronika - Fojta, Miroslav - Daňhel, Aleš
    Constant-current chronopotentiometric stripping detection of bovine serum albumin on silver amalgam particles.
    Journal of Electroanalytical Chemistry. Roč. 859, FEB 15 2020 (2020), č. článku 113854. ISSN 1572-6657. E-ISSN 1873-2569
    Grant CEP: GA ČR(CZ) GJ17-23634Y; GA MŠk EF15_003/0000477
    Institucionální podpora: RVO:68081707
    Klíčová slova: protein * voltammetry * Bovine serum albumin
    Kód oboru RIV: CG - Elektrochemie
    Obor OECD: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
    Impakt faktor: 4.464, rok: 2020
    https://www.sciencedirect.com/science/article/abs/pii/S1572665720300370

    Highly sensitive peak H registered by constant-current chronopotentiometric stripping (CPS) analysis relates due to the catalytic hydrogen evolution reaction of proteins adsorbed on mercury-based surfaces including recently introduced silver amalgam particles (AgAPs). Since the peak H allows a structure-sensitive analysis of proteins, including those important in biomedicine and cancer research, it has been systematically studied by CPS analysis using AgAPs in this work. Conditions of AgAPs' electrodeposition on basal plane pyrolytic graphite electrode (bPGE) were optimized towards CPS analysis of model protein (bovine serum albumin, BSA) using peak H. Optimized condition for bPGE-AgAP preparation allowed sensitive CPS detection of either native or denatured BSA down to 10 nM concentration, a discrimination between both forms, and registration of the BSA thermal denaturation kinetic. Obtained results have confirmed the applicability of AgAPs in protein electroanalysis and may contribute to the further development of novel (bio)sensors or (bio)electrochemical applications using micro-volume batch, flow-through or lab-on-chip detection systems. (C) 2020 Elsevier B.V. All rights reserved.
    Trvalý link: http://hdl.handle.net/11104/0308622

     
     
Počet záznamů: 1