The high-resolution proteomic analysis of protein composition of rat spleen lymphocytes stimulated by Concanavalin A, a comparison with morphine-treated cells

0523837 - FGÚ 2021 RIV NL eng J - Článek v odborném periodiku
Ujčíková, Hana - Cechová, Kristína - Roubalová, Lenka - Brejchová, Jana - Kaufman, Jonáš - Holáň, Vladimír - Svoboda, Petr
The high-resolution proteomic analysis of protein composition of rat spleen lymphocytes stimulated by Concanavalin A, a comparison with morphine-treated cells.
Journal of Neuroimmunology. Roč. 341, Apr 15 (2020), č. článku 577191. ISSN 0165-5728. E-ISSN 1872-8421
Grant CEP: GA ČR(CZ) GA19-03295S; GA MŠMT(CZ) LTAUSA18110
Institucionální podpora: RVO:67985823 ; RVO:68378041
Klíčová slova: morphine * concanavalin A * rat spleen lymphocytes * proteomic analysis * up-regulation of brain proteins
Obor OECD: Physiology (including cytology); Biochemistry and molecular biology (UEM-P)
Impakt faktor: 3.478, rok: 2020
Způsob publikování: Omezený přístup
https://doi.org/10.1016/j.jneuroim.2020.577191

Morphine- and Concanavalin A-induced changes of protein composition of rat spleen lymphocytes were determined by high-resolution proteomic analysis, gel-free, label-free quantification, MaxLFQ. Stimulation by Con A resulted in a major reorganization of spleen cell protein composition evidenced by increased expression level of 94 proteins, 101 proteins were down-regulated (> 2-fold). Interestingly, among proteins that were up-regulated to the largest extent were the prototypical brain proteins as a neuron specific enolase, synapsin-1, brain acid-soluble protein-1 and myelin basic protein. Morphine-induced change was limited to no more than 5 up-regulated and 18 down-regulated proteins (> 2-fold).
Trvalý link: http://hdl.handle.net/11104/0308117