Počet záznamů: 1
Pseudogemmobacter bohemicus gen. nov., sp. nov., a novel taxon from the Rhodobacteraceae family isolated from heavy-metal-contaminated sludge
- 1.0522843 - ÚMG 2020 RIV GB eng J - Článek v odborném periodiku
Šuman, J. - Zubrová, A. - Rojíková, K. - Pechar, R. - Švec, P. - Cajthaml, T. - Ulbrich, P. - Rídl, Jakub - Strnad, Hynek - Uhlík, O.
Pseudogemmobacter bohemicus gen. nov., sp. nov., a novel taxon from the Rhodobacteraceae family isolated from heavy-metal-contaminated sludge.
International Journal of Systematic and Evolutionary Microbiology. Roč. 69, č. 8 (2019), s. 2401-2407. ISSN 1466-5026
Institucionální podpora: RVO:68378050
Klíčová slova: Rhodobacteraceae * Pseudogemmobacter bohemicus * 16S rRNA gene analysis * chemotaxonomic characterization * percentage of conserved proteins * heavy metals
Kód oboru RIV: EB - Genetika a molekulární biologie
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 2.415, rok: 2019
The creamy white to beige, aerobic, non-motile, ovoid to rod-shaped, Gram-stain-negative strain, Cd-10(T), was isolated from heavy-metal-contaminated sludge from a decantation basin of a heavy metal processing factory based on its ability to tolerate CdCl2 in the cultivation medium. In the reconstruction of its phylogeny based on 16S rRNA gene sequences, strain Cd-10(T) clustered with species of the genera Gemmobacter, Xinfangfangia, Tabrizicola and Rhodobacter within the family Rhodobacteraceae. Its 16S rRNA gene sequence exhibited 96.32% pairwise similarity to the type strain of Xinfangfangia soli, 95.3 % to that of Gemmobacter intermedius, followed by Tabrizicola fusiformis (95.10 %), Rhodobacter sediminis (94.88 %), Gemmobacter nectariphilus and Rhodobacter capsulatus (both 94.81%). The major respiratory quinone was Q-10 accompanied by Q-9, the fatty acid profile consisted predominantly of C-18:1 omega 7C, C-18:0, C-16:0 and C-16:1 omega 7c, the major polar lipids were phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine and diphosphatidylglycerol. An analysis of the percentage of conserved proteins deduced from draft or complete genomic sequences of strain Cd-10(T) and representatives of its closest relatives suggested that strain Cd-10(T) is a member of a novel genus within the Rhodobacteraceae family for which we propose the name Pseudogemmobacter. Strain Cd-10(T) (=DSM 103618(T)=NCCB 100645(T)) is the type strain of Pseudogemmobacter bohemicus gen. nov., sp. nov., the type species of the genus Pseudogemmobacter gen. nov.
Trvalý link: http://hdl.handle.net/11104/0307265
Počet záznamů: 1