The 3D imaging of mesenchymal stem cells on porous scaffolds using high-contrasted x-ray computed nanotomography

0518846 - ÚEM 2020 RIV GB eng J - Článek v odborném periodiku
Vojtová, L. - Zikmund, T. - Pavliňáková, V. - Šalplachta, J. - Kalašová, D. - Prosecká, Eva - Brtníková, J. - Žídek, J. - Pavliňák, D. - Kaiser, J.
The 3D imaging of mesenchymal stem cells on porous scaffolds using high-contrasted x-ray computed nanotomography.
Journal of Microscopy. Roč. 273, č. 3 (2019), s. 169-177. ISSN 0022-2720. E-ISSN 1365-2818
Institucionální podpora: RVO:68378041
Klíčová slova: biopolymeric scaffold * mesenchymal stem cells * SEM/EDX
Obor OECD: Biomaterials (as related to medical implants, devices, sensors)
Impakt faktor: 1.575, rok: 2019
Způsob publikování: Omezený přístup
https://onlinelibrary.wiley.com/doi/abs/10.1111/jmi.12771

This study presents an X-ray computed nanotomography (nano-CT) based, high-resolution imaging technique. Thanks to a voxel resolution of 540 nm, this novel technique is suitable for observing the 3D morphology of soft biopolymeric scaffolds seeded with stem cells. A sample of highly porous collagen scaffold seeded with contrasted mesenchymal stem cells (MSC) was investigated by using lab-based nano-CT. The whole volume of the sample was analysed without its destruction. To evaluate the potential of nano-CT, a comparison measurement was done using a standard microscopy technique. Scanning electron microscopy (SEM) combined with energy dispersive X-ray analysis (EDX) established an extension and local accumulation of the contrasting agent - heavy metallic osmium tetroxide. The presented imaging technique is novel as it will help to understand better the behaviour of cells while interacting with three-dimensional biomaterials. This is crucial for both experimental and clinical tissue engineering applications in order to limit the risk of uncontrolled cell growth, and potentially tumour formation.
Trvalý link: http://hdl.handle.net/11104/0303874