0518091 - ÚOCHB 2020 RIV eng P - Patentový dokument
Šácha, Pavel - Konvalinka, Jan - Schimer, Jiří - Knedlík, Tomáš - Šubr, Vladimír - Ulbrich, Karel - Strohalm, Jiří
Macromolecular conjugates for isolation, immobilization and visualization of proteins.
2019. Vlastník: Ústav organické chemie a biochemie AV ČR, v. v. i. - Ústav makromolekulární chemie AV ČR, v. v. i. - Univerzita Karlova v Praze. Datum udělení patentu: 26.11.2019. Číslo patentu: CA2970845
Grant CEP: GA ČR(CZ) GA16-02938S; GA MŠMT LO1302; GA MZd(CZ) NV15-31379A
Institucionální podpora: RVO:61388963 ; RVO:61389013
Klíčová slova: antibody mimetics * polymer * His tag
Obor OECD: Biochemistry and molecular biology; Polymer science (UMCH-V)
Web výsledku:
https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=CA&NR=2970845C&KC=C&FT=D&ND=6&date=20191126&DB=&locale=en_EP#

Macromolecular conjugates for isolation, immobilization and visualization of proteins The invention provides a synthetic macromolecular conjugate for selective interaction with proteins, comprising a synthetic copolymer, and at least one binding group and at least one further group selected from an affinity tag and an imaging probe, said at least one binding group and at least one further group being bound via covalent bond to said synthetic copolymer. The macromolecular conjugate is suitable in particular for identification, visualization, quantification or isolation of proteins and/ or cells. HPMA copolymer, i.e.poly(HPMA-co-Ma-ß-Ala-TT), a copolymer prepared by conventional solution radical polymerization or controlled radical copolymerization (e.g. RAFT copolymerization, reversible addition-fragmentation chain-transfer) of N-(2- hydroxypropyl) methacrylamide (HPMA) and 3-(3-methacrylamidopropanoyl) thiazolidine-2-thione (Ma-P-Ala-TT) can be preferably used as the basic copolymer.
Trvalý link: http://hdl.handle.net/11104/0303277