Počet záznamů: 1
Activity and inactivity of moth sex chromosomes in somatic and meiotic cells
- 1.0507648 - BC 2020 RIV US eng J - Článek v odborném periodiku
Traut, W. - Schubert, V. - Dalíková, Martina - Marec, František - Sahara, K.
Activity and inactivity of moth sex chromosomes in somatic and meiotic cells.
Chromosoma. Roč. 128, č. 4 (2019), s. 533-545. ISSN 0009-5915. E-ISSN 1432-0886
Grant CEP: GA ČR(CZ) GA17-13713S
Grant ostatní: GA ČR(CZ) GA17-17211S
Institucionální podpora: RVO:60077344
Klíčová slova: heterochromatin * sex chromatin * germline-limited activity
Obor OECD: Genetics and heredity (medical genetics to be 3)
Impakt faktor: 3.442, rok: 2019
Způsob publikování: Omezený přístup
https://link.springer.com/article/10.1007%2Fs00412-019-00722-8
Moths and butterflies (Lepidoptera) are the most species-rich group of animals with female heterogamety, females mostly having a WZ, males a ZZ sex chromosome constitution. We studied chromatin conformation, activity, and inactivity of the sex chromosomes in the flour moth Ephestia kuehniella and the silkworm Bombyx mori, using immunostaining with anti-H3K9me2/3, anti-RNA polymerase II, and fluoro-uridine (FU) labelling of nascent transcripts, with conventional widefield fluorescence microscopy and 'spatial structured illumination microscopy' (3D-SIM). The Z chromosome is euchromatic in somatic cells and throughout meiosis. It is transcriptionally active in somatic cells and in the postpachaytene stage of meiosis. The W chromosome in contrast is heterochromatic in somatic cells as well as in meiotic cells at pachytene, but euchromatic and transcriptionally active like all other chromosomes at postpachytene. As the W chromosomes are apparently devoid of protein-coding genes, their transcripts must be non-coding. We found no indication of 'meiotic sex chromosome inactivation' (MSCI) in the two species.
Trvalý link: http://hdl.handle.net/11104/0303635
Počet záznamů: 1