Poly(D,L-lactide)/polyethylene glycol micro/nanofiber mats as paclitaxel-eluting carriers: preparation and characterization of fibers, in vitro drug release, antiangiogenic activity and tumor recurrence prevention

0500120 - ÚMCH 2020 RIV NL eng J - Článek v odborném periodiku
Hobzová, Radka - Hampejsová, Z. - Černá, T. - Hraběta, J. - Venclíková, Kristýna - Jedelská, J. - Bakowsky, U. - Bosáková, Z. - Lhotka, M. - Vaculín, Š. - Franěk, M. - Steinhart, Miloš - Kovářová, Jana - Michálek, Jiří - Širc, Jakub
Poly(D,L-lactide)/polyethylene glycol micro/nanofiber mats as paclitaxel-eluting carriers: preparation and characterization of fibers, in vitro drug release, antiangiogenic activity and tumor recurrence prevention.
Materials Science & Engineering C-Materials for Biological Applications. Roč. 98, May (2019), s. 982-993. ISSN 0928-4931. E-ISSN 1873-0191
Grant CEP: GA ČR(CZ) GA16-04863S; GA MŠMT(CZ) LQ1604; GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:61389013
Klíčová slova: PLA/PEG micro/nanofibers * needleless electrospinning * paclitaxel quantification
Obor OECD: Polymer science
Impakt faktor: 5.880, rok: 2019
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/pii/S0928493118308683?via%3Dihub

Poly(d,l-lactide)/polyethylene glycol (PLA/PEG) micro/nanofibers loaded with paclitaxel (PTX, 10 wt%) were prepared by needless electrospinning technology, which allows large scale production for real medicinal practice. The fiber structure and properties were investigated by several methods including scanning electron microscopy, nitrogen adsorption/desorption isotherm measurements, differential scanning calorimetry, and X-ray diffraction measurements to examine their morphology (fiber diameter distribution, specific surface area, and total pore volume), composition, drug-loading efficiency, and physical state. An HPLC-UV method was optimized and validated to quantify in vitro PTX release into PBS. The results showed that the addition of PEG into PLA fibers promoted the release of higher amounts of hydrophobic PTX over prolonged time periods compared to fibers without PEG. An in vitro cell assay demonstrated the biocompatibility of PLA/PEG fibrous materials and showed significant cytotoxicity of PTX-loaded PLA/PEG fibers against a human fibrosarcoma HT1080 cell line. The chick chorioallantoic membrane assay proved that PTX-loaded fibers exhibited antiangiogenic activity, with a pronounced effect in the case of the PEG-containing fibers. In vivo evaluation of PTX-loaded PLA/PEG fibers in a human fibrosarcoma recurrence model showed statistically significant inhibition in tumor incidence and growth after primary tumor resection compared to other treatment groups.
Trvalý link: http://hdl.handle.net/11104/0292620