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´Candidatus Phytoplasma asteris´and ´Candidatus Phytoplasma mali´ strains infecting sweet and sour cherry in the Czech Republic

  1. 1.
    0495075 - BC 2019 RIV DE eng J - Článek v odborném periodiku
    Fránová, Jana - Lenz, Ondřej - Přibylová, Jaroslava - Špak, Josef - Koloniuk, Igor - Suchá, J. - Paprstein, F.
    ´Candidatus Phytoplasma asteris´and ´Candidatus Phytoplasma mali´ strains infecting sweet and sour cherry in the Czech Republic.
    Journal of Phytopathology - Phytopathologische Zeitschrift. Roč. 166, č. 1 (2018), s. 59-66. ISSN 0931-1785
    Grant CEP: GA MŠk(CZ) LD14004
    Institucionální podpora: RVO:60077344
    Klíčová slova: 16S rRNA gene * PCR/RFLP * phylogeny * Prunus
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Obor OECD: Plant sciences, botany
    Impakt faktor: 1.097, rok: 2018

    A survey for phytoplasma diseases was conducted in a sweet and sour cherry germplasm collection and in cherry orchards within the Czech Republic during 2014-2015. Phytoplasmas were detected in 21 symptomatic trees. Multiple infections of cherry trees by diverse phytoplasmas of 16SrI and 16SrX groups and 16SrI-A, 16SrI-B, 16SrI-L, 16SrX-A subgroups were detected by restriction fragment length polymorphism (RFLP). Nevertheless, phylogenetic analysis placed subgroups 16SrI-B and 16SrI-L inseparable together onto one branch of phylogenetic tree. This is the first report of subgroups 16SrI-A and 16SrI-L in Prunus spp., and subgroup 16SrX-A in sour cherry trees. Additionally, novel RFLP profiles for 16SrI-A and 16SrI-B-related phytoplasmas were found in cherry samples. Phytoplasmas with these novel profiles belong, however, to their respective 16SrI-A or 16SrI-B phylogenetic clades.
    Trvalý link: http://hdl.handle.net/11104/0288094
     
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