Počet záznamů: 1  

Differential modulation of white adipose tissue endocannabinoid levels by n-3 fatty acids in obese mice and type 2 diabetic patients

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    0493048 - FGU-C 2019 RIV NL eng J - Článek v odborném periodiku
    Rossmeisl, Martin - Pavlišová, Jana - Janovská, Petra - Kuda, Ondřej - Bardová, Kristina - Hansíková, Jana - Svobodová, Michaela - Oseeva, Marina - Veleba, J. - Kopecký Jr., J. - Žáček, P. - Fišerová, E. - Pelikánová, T. - Kopecký, Jan
    Differential modulation of white adipose tissue endocannabinoid levels by n-3 fatty acids in obese mice and type 2 diabetic patients.
    Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids. Roč. 1863, č. 7 (2018), s. 712-725. ISSN 1388-1981
    Grant CEP: GA ČR(CZ) GA14-09347S; GA MZd(CZ) NV15-27431A
    Institucionální podpora: RVO:67985823
    Klíčová slova: 2-AG * DHEA * high-fat diet * obesity * Omega-3 PUFA
    Kód oboru RIV: FB - Endokrinologie, diabetologie, metabolizmus, výživa
    Obor OECD: Endocrinology and metabolism (including diabetes, hormones)
    Impakt faktor: 4.402, rok: 2018

    n-3 polyunsaturated fatty acids (n-3 PUFA) might regulate metabolism by lowering endocannabinoid levels. We examined time-dependent changes in adipose tissue levels of endocannabinoids as well as in parameters of glucose homeostasis induced by n-3 PUFA in dietary-obese mice, and compared these results with the effect of n-3 PUFA intervention in type 2 diabetic (T2DM) subjects. Male C57BL/6J mice were fed for 8, 16 or 24 weeks a high-fat diet alone (cHF) or supplemented with n-3 PUFA (cHF + F). Overweight/obese, T2DM patients on metformin therapy were given for 24 weeks corn oil (Placebo, 5 g/day) or n-3 PUFA concentrate as above (Omega-3, 5 g/day). Endocannabinoids were measured by liquid chromatography-tandem mass-spectrometry. Compared to cHF-fed controls, the cHF + F mice consistently reduced 2-arachidonoylglycerol (up to similar to 2-fold at week 24) and anandamide (similar to 2-fold) in adipose tissue, while the levels of endocannabinoid-related anti-inflammatory molecules N-eicosapentaenoyl ethanolamine (EPEA) and N-docosahexaenoyl ethanolamine (DHEA) increased more than similar to 10-fold and similar to 8-fold, respectively. At week 24, the cHF + F mice improved glucose tolerance and fasting blood glucose, the latter being positively correlated with adipose 2-arachidonoylglycerol levels only in obese cHF-fed controls, like fasting insulin and HOMA-IR. In the patients, n-3 PUFA failed to reduce 2-arachidonoylglycerol and anandamide levels in adipose tissue and serum, but they increased both adipose tissue and serum levels of EPEA and DHEA. In conclusion, the inability of n-3 PUFA to reduce adipose tissue and serum levels of classical endocannabinoids might contribute to a lack of beneficial effects of these lipids on glucose homeostasis in T2DM patients.
    Trvalý link: http://hdl.handle.net/11104/0286517