0491033 - ÚIACH 2019 SK eng A - Abstrakt
Šlampová, Andrea - Kubáň, Pavel
Two-phase micro-electromembrane extraction across free liquid membrane for determination of acidic drugs in complex samles.
Book of abstracts. 24th International Symposium on Separation Sciences (ISSS 2018). Bratislava: Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University in Bratislava, 2018 - (Hutta, M.; Bodor, R.; Halko, R.). s. 170-170. ISBN 978-80-971179-8-6.
[International Symposium on Separation Sciences /24./ - ISSS 2018, International Conference Analytical Methods and Human Health /21./ - AMHH 2018. 17.06.2018-20.06.2018, Jasná]
Grant CEP: GA ČR(CZ) GA16-09135S
Institucionální podpora: RVO:68081715
Klíčová slova: micro-electromembrane extraction * capillary electrophoresis * acidic drugs
Obor OECD: Analytical chemistry

In this contribution a novel two-phase μ-EME using electrically induced transfer of charged analytes directly into FLM was proposed as an effective technique for improving enrichment capabilities of μ-EME. The presented set-up employs aqueous donor solution and water immiscible organic solvent, which form two-phase μ-EME system, for efficient extraction of acidic drugs from human body fluids and waste water samples. The organic solvent is filled in a micropipette tip, which is immersed into the complex sample, and forms the FLM. The FLM efficiently eliminates migration of matrix components from sample solution and simultaneously it acts as an acceptor solution for selective trapping and enrichment of analyte ions. Electrodes are inserted directly into the sample and the FLM and replenishment of analyte ions at the sample/FLM phase interface is accomplished by stirring the sample solution using a conventional
laboratory stirrer. Various volumetric ratios of both phases were tested for efficient transfer and enrichment of acidic drugs. At optimized two-phase μ-EME conditions and at volume ratio of sample to FLM (480 to 16 μL), extraction recovery 75 – 95% and enrichment factors up to 28 were achieved for ibuprofen, ketoprofen, naproxen and diclofenac. Determination of the acidic drugs in resulting FLMs was achieved by capillary electrophoresis with ultraviolet detection. Analysis of selected acidic drugs in human urine and waste water is also demonstrated.
Trvalý link: http://hdl.handle.net/11104/0285108