Počet záznamů: 1  

The Biological Role of Hyaluronan-Rich Oocyte-Cumulus Extracellular Matrix in Female Reproduction

  1. 1.
    0489476 - UZFG-Y 2019 RIV CH eng J - Článek v odborném periodiku
    Nagyová, Eva
    The Biological Role of Hyaluronan-Rich Oocyte-Cumulus Extracellular Matrix in Female Reproduction.
    International Journal of Molecular Sciences. Roč. 19, č. 1 (2018), č. článku 283. E-ISSN 1422-0067
    Grant CEP: GA MŠk EF15_003/0000460
    Institucionální podpora: RVO:67985904
    Klíčová slova: extracellular matrix * hyaluronan * inter-alpha-trypsin inhibitor
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Obor OECD: Biochemistry and molecular biology
    Impakt faktor: 4.183, rok: 2018

    Fertilization of the mammalian oocyte requires interactions between spermatozoa and expanded cumulus extracellular matrix (ECM) that surrounds the oocyte. This review focuses on key molecules that play an important role in the formation of the cumulus ECM, generated by the oocyte-cumulus complex. In particular, the specific inhibitors (AG1478, lapatinib, indomethacin and MG132) and progesterone receptor antagonist (RU486) exerting their effects through the remodeling of the ECM of the cumulus cells surrounding the oocyte have been described. After gonadotropin stimulus, cumulus cells expand and form hyaluronan (HA)-rich cumulus ECM. In pigs, the proper structure of the cumulus ECM depends on the interaction between HA and serum-derived proteins of the inter-alpha-trypsin inhibitor (II) protein family. We have demonstrated the synthesis of HA by cumulus cells, and the presence of the II, tumor necrosis factor-alpha-induced protein 6 and pentraxin 3 in expanding oocyte-cumulus complexes (OCC). We have evaluated the covalent linkage of heavy chains of II proteins to HA, as the principal component of the expanded HA-rich cumulus ECM, in porcine OCC cultured in medium with specific inhibitors: AG1478 and lapatinib (both inhibitors of epidermal growth factor receptor tyrosine kinase activity), MG132 (a specific proteasomal inhibitor), indomethacin (cyclooxygenase inhibitor), and progesterone receptor antagonist (RU486). We have found that both RU486 and indomethacin does not disrupt the formation of the covalent linkage between the heavy chains of II to HA in the expanded OCC. In contrast, the inhibitors AG1478 and lapatinib prevent gonadotropin-induced cumulus expansion. Finally, the formation of oocyte-cumulus ECM relying on the covalent transfer of heavy chains of II molecules to HA has been inhibited in the presence of MG132.
    Trvalý link: http://hdl.handle.net/11104/0283885