Počet záznamů: 1  

Bordetella pertussis Adenylate Cyclase Toxin Disrupts Functional Integrity of Bronchial Epithelial Layers

  1. 1.
    0488787 - MBU-M 2019 RIV US eng J - Článek v odborném periodiku
    Hasan, Shakir - Kulkarni, N.N. - Asbjarnarson, A. - Linhartová, Irena - Osička, Radim - Šebo, Peter - Gudmundsson, H.
    Bordetella pertussis Adenylate Cyclase Toxin Disrupts Functional Integrity of Bronchial Epithelial Layers.
    Infection and Immunity. Roč. 86, č. 3 (2018), č. článku e00445-17. ISSN 0019-9567
    Grant CEP: GA ČR GA15-09157S; GA MZd(CZ) NV16-28126A; GA MŠk(CZ) LM2015064; GA ČR(CZ) GA18-20621S; GA MŠk(CZ) EF16_013/0001818
    Institucionální podpora: RVO:61388971
    Klíčová slova: Bordetella pertussis * airway epithelia * CyaA
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Obor OECD: Microbiology
    Impakt faktor: 3.160, rok: 2018

    The airway epithelium restricts the penetration of inhaled pathogens into the underlying tissue and plays a crucial role in the innate immune defense against respiratory infections. The whooping cough agent, Bordetella pertussis, adheres to ciliated cells of the human airway epithelium and subverts its defense functions through the action of secreted toxins and other virulence factors. We examined the impact of B. pertussis infection and of adenylate cyclase toxin-hemolysin (CyaA) action on the functional integrity of human bronchial epithelial cells cultured at the air-liquid interface (ALI). B. pertussis adhesion to the apical surface of polarized pseudostratified VA10 cell layers provoked a disruption of tight junctions and caused a drop in transepithelial electrical resistance (TEER). The reduction of TEER depended on the capacity of the secreted CyaA toxin to elicit cAMP signaling in epithelial cells through its adenylyl cyclase enzyme activity. Both purified CyaA and cAMP-signaling drugs triggered a decrease in the TEER of VA10 cell layers. Toxin-produced cAMP signaling caused actin cytoskeleton rearrangement and induced mucin 5AC production and interleukin-6 (IL-6) secretion, while it inhibited the IL-17A-induced secretion of the IL-8 chemokine and of the antimicrobial peptide beta-defensin 2. These results indicate that CyaA toxin activity compromises the barrier and innate immune functions of Bordetella-infected airway epithelia.
    Trvalý link: http://hdl.handle.net/11104/0283351